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Literature summary extracted from

  • Degrassi, G.; Okeke, B.C.; Bruschi, C.V.; Venturi, V.
    Purification and characterization of an acetyl xylan esterase from Bacillus pumilus (1998), Appl. Environ. Microbiol., 64, 789-792.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.6 Cu2+
-
 Bacillus pumilus
3.1.1.6 Fe2+
-
 Bacillus pumilus
3.1.1.6 Zn2+
-
 Bacillus pumilus
3.1.1.72 Ag+
-
 Bacillus pumilus
3.1.1.72 Ca2+
-
 Bacillus pumilus
3.1.1.72 Co2+
-
 Bacillus pumilus
3.1.1.72 Cu2+
-
 Bacillus pumilus
3.1.1.72 Fe2+
-
 Bacillus pumilus
3.1.1.72 Mn2+
-
 Bacillus pumilus
3.1.1.72 Zn2+
-
 Bacillus pumilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.6 1.54
-
 1-naphthol acetate
-
 Bacillus pumilus
3.1.1.72 1.54
-
 1-naphthyl acetate
-
 Bacillus pumilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.72 extracellular
-
 Bacillus pumilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.6 40000
-
 x * 40000, SDS-PAGE Bacillus pumilus
3.1.1.6 190000
-
 gel filtration Bacillus pumilus
3.1.1.72 40000
-
 4 * or 5 * 40000, SDS-PAGE Bacillus pumilus
3.1.1.72 40000
-
 5 * or 4 * 40000, SDS-PAGE Bacillus pumilus
3.1.1.72 190000
-
 gel filtration Bacillus pumilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.72 additional information Bacillus pumilus induced by corncob powder and xylan ?
-
 ?
3.1.1.72 additional information Bacillus pumilus PS214 induced by corncob powder and xylan ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.6 Bacillus pumilus
-
-
-
3.1.1.72 Bacillus pumilus
-
-
-
3.1.1.72 Bacillus pumilus PS214
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.6
-
 Bacillus pumilus
3.1.1.72
-
 Bacillus pumilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.6 118.8
-
-
 Bacillus pumilus
3.1.1.72 118.8
-
-
 Bacillus pumilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.6 1-naphthol acetate + H2O
-
 Bacillus pumilus 1-naphthol + acetate
-
 ?
3.1.1.6 4-methylumbelliferyl acetate + H2O
-
 Bacillus pumilus ?
-
 ?
3.1.1.6 acetylxylan + H2O
-
 Bacillus pumilus ?
-
 ?
3.1.1.6 glucose pentaacetate + H2O
-
 Bacillus pumilus ?
-
 ?
3.1.1.6 p-nitrophenyl acetate + H2O
-
 Bacillus pumilus p-nitrophenol + acetate
-
 ?
3.1.1.6 xylose tetraacetate + H2O
-
 Bacillus pumilus ?
-
 ?
3.1.1.72 1-naphthyl acetate + H2O
-
 Bacillus pumilus naphthol + acetate
-
 ?
3.1.1.72 1-naphthyl acetate + H2O
-
 Bacillus pumilus PS214 naphthol + acetate
-
 ?
3.1.1.72 4-methylumbelliferyl acetate
-
 Bacillus pumilus acetate + 4-methylumbelliferol
-
 ?
3.1.1.72 4-methylumbelliferyl acetate
-
 Bacillus pumilus PS214 acetate + 4-methylumbelliferol
-
 ?
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Bacillus pumilus 4-nitrophenol + acetate
-
 ?
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Bacillus pumilus PS214 4-nitrophenol + acetate
-
 ?
3.1.1.72 acetylxylan
-
 Bacillus pumilus acetate + ?
-
 ?
3.1.1.72 acetylxylan
-
 Bacillus pumilus PS214 acetate + ?
-
 ?
3.1.1.72 additional information induced by corncob powder and xylan Bacillus pumilus ?
-
 ?
3.1.1.72 additional information induced by corncob powder and xylan Bacillus pumilus PS214 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.6 multimer x * 40000, SDS-PAGE Bacillus pumilus
3.1.1.72 pentamer 5 * or 4 * 40000, SDS-PAGE Bacillus pumilus
3.1.1.72 tetramer 4 * or 5 * 40000, SDS-PAGE Bacillus pumilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.6 55
-
-
 Bacillus pumilus
3.1.1.72 55
-
-
 Bacillus pumilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.72 50
-
 stable Bacillus pumilus
3.1.1.72 60
-
 half-life: 1 h Bacillus pumilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.6 8
-
-
 Bacillus pumilus
3.1.1.72 8
-
-
 Bacillus pumilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.72 8 9.5 stable Bacillus pumilus