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Literature summary extracted from

  • Tamada, Y.; Swanson, B.A.; Arabshahi, A.; Frey, P.A.
    Preparation and characterization of a bifunctional fusion enzyme composed of UDP-galactose 4-epimerase and galactose-1-P uridylyltransferase (1994), Bioconjug. Chem., 5, 660-665.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.3.2 synthesis preparation of a fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker, shows kinetic advantages in that the initial velocity to produce glucose 1-phosphate from UDPgalactose an Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.12 construction and overexpression of bifunctional fusion protein composed of galactose-2-phosphate uridylyltransferase and UDP-galactose 4-epimerase with an intervening linker of 3 Ala residues Escherichia coli
5.1.3.2 fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.12 additional information construction and expression of bifunctional fusion protein composed of galactose-2-phosphate uridylyltransferase and UDP-galactose 4-epimerase with an intervening linker of 3 Ala residues, 20% increased Vmax compared to a mixture of the single enzymes Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.12 0.08
-
 UDP-glucose purified recombinant bifunctional chimeric fusion protein, pH 8.5, 27°C Escherichia coli
2.7.7.12 0.29
-
 alpha-D-galactose 1-phosphate purified recombinant bifunctional chimeric fusion protein, pH 8.5, 27°C Escherichia coli
5.1.3.2 0.14
-
 UDPgalactose fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.12 additional information
-
 the recombinant chimeric fusion protein exists as monomer, dimer and tetramer, all are active Escherichia coli
2.7.7.12 80000
-
 recombinant bifunctional chimeric fusion protein, monomer, gel filtration Escherichia coli
5.1.3.2 80000
-
 2 * 80000, fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker exist as monomeric, dimeric and tetrameric form. The monomeric form has low epimerase activity, SDS-PAGE Escherichia coli
5.1.3.2 80000
-
 4 * 80000, fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker exist as monomeric, dimeric and tetrameric form. The monomeric form has low epimerase activity, SDS-PAGE Escherichia coli
5.1.3.2 160000
-
 fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker, dimeric form, gel filtration Escherichia coli
5.1.3.2 320000
-
 fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker, tetrameric form, gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Escherichia coli
-
 galT gene
-
5.1.3.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12 recombinant bifunctional fusion protein from strain BL21(DE3), pLysS, pT7ET to near homogeneity, 9fold Escherichia coli
5.1.3.2 fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.12 1080
-
 purified recombinant bifunctional chimeric fusion protein Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate recombinant bifunctional chimeric fusion protein Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
 r
5.1.3.2 UDP-galactose
-
 Escherichia coli UDP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.12 More the recombinant chimeric fusion protein exists as monomer, dimer and tetramer, all are active Escherichia coli
5.1.3.2 dimer 2 * 80000, fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker exist as monomeric, dimeric and tetrameric form. The monomeric form has low epimerase activity, SDS-PAGE Escherichia coli
5.1.3.2 monomer 2 * 80000, fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker exist as monomeric, dimeric and tetrameric form. The monomeric form has low epimerase activity, SDS-PAGE Escherichia coli
5.1.3.2 tetramer 4 * 80000, fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker exist as monomeric, dimeric and tetrameric form. The monomeric form has low epimerase activity, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.12 24
-
 alpha-D-galactose 1-phosphate purified recombinant bifunctional chimeric fusion protein, pH 8.5, 27°C Escherichia coli
5.1.3.2 960
-
 UDPgalactose fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker Escherichia coli