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Literature summary extracted from

  • Adams, E.
    Enzymes and intermediates of hydroxyproline degradation (1971), Methods Enzymol., 17B, 266-306.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.16 glyoxylate
-
 Rattus norvegicus
4.1.3.16 pyruvate
-
 Rattus norvegicus
5.1.1.8 3-Pyrrolidinol
-
 Pseudomonas putida
5.1.1.8 pyrrole-2-carboxylate
-
 Pseudomonas putida
5.1.1.8 sarcosine
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.16 0.11
-
 D-4-hydroxy-2-oxoglutarate
-
 Rattus norvegicus
4.1.3.16 0.22
-
 L-4-hydroxy-2-oxoglutarate
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.16 additional information no requirement Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.16 120000
-
 gel filtration Rattus norvegicus
5.1.1.8 18000
-
 approach to-equilibrium method Pseudomonas putida
5.1.1.8 44000
-
 gel filtration Pseudomonas putida
5.1.1.8 60000
-
 analytical ultracentrifugation Pseudomonas putida
5.1.1.8 64000
-
 ultracentrifugal data, study of tryptic peptides and amino acid composition, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.16 4-hydroxy-2-oxoglutarate Rattus norvegicus
-
 ?
-
 ?
5.1.1.8 Hydroxy-L-Pro Pseudomonas putida enzyme in pathway of hydroxyproline oxidation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.16 Rattus norvegicus
-
 rat
-
5.1.1.8 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.16
-
 Rattus norvegicus
5.1.1.8
-
 Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
5.1.1.8 trans-4-Hydroxy-L-proline = cis-4-hydroxy-D-proline a single hydrogen of 4-hydroxyproline is labilized and exchanges with H2O in the medium Pseudomonas putida
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.16 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.16 8
-
-
 Rattus norvegicus
5.1.1.8 164
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.16 4-Hydroxy-2-oxoglutarate both stereoisomers Rattus norvegicus Pyruvate + glyoxylate
-
 ?
4.1.3.16 4-hydroxy-2-oxoglutarate
-
 Rattus norvegicus ?
-
 ?
4.1.3.16 D-4-hydroxy-2-oxoglutarate
-
 Rattus norvegicus pyruvate + glyoxylate
-
 ?
4.1.3.16 L-4-hydroxy-2-oxoglutarate
-
 Rattus norvegicus pyruvate + glyoxylate
-
 ?
4.1.3.16 additional information acts on both stereoisomers Rattus norvegicus ?
-
 ?
4.1.3.16 additional information 2-keto-3-deoxyglucarate and 2-keto-4,5-dihydroxyvalerate cleaved at slow rate Rattus norvegicus ?
-
 ?
5.1.1.8 Hydroxy-L-Pro enzyme in pathway of hydroxyproline oxidation Pseudomonas putida ?
-
 ?
5.1.1.8 trans-4-hydroxy-L-Pro
-
 Pseudomonas putida cis-4-hydroxy-D-Pro
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.16 37
-
 enzyme assay at Rattus norvegicus