EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.1.1.8 | additional information | pyridoxal 5'-phosphate is not involved in the reaction | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.1.8 | 1,10-phenanthroline | - |
Pseudomonas putida | |
5.1.1.8 | 2,2'-dipyridyl | - |
Pseudomonas putida | |
5.1.1.8 | Acriflavin | - |
Pseudomonas putida | |
5.1.1.8 | AgNO3 | - |
Pseudomonas putida | |
5.1.1.8 | Atebrin | - |
Pseudomonas putida | |
5.1.1.8 | CdCl2 | - |
Pseudomonas putida | |
5.1.1.8 | CuSO4 | - |
Pseudomonas putida | |
5.1.1.8 | HgCl2 | - |
Pseudomonas putida | |
5.1.1.8 | hydrazine | - |
Pseudomonas putida | |
5.1.1.8 | hydroxylamine | - |
Pseudomonas putida | |
5.1.1.8 | iodoacetate | - |
Pseudomonas putida | |
5.1.1.8 | KCN | - |
Pseudomonas putida | |
5.1.1.8 | NaAsO2 | - |
Pseudomonas putida | |
5.1.1.8 | NEM | - |
Pseudomonas putida | |
5.1.1.8 | p-chloromercuribenzoate | - |
Pseudomonas putida | |
5.1.1.8 | Semicarbazide | - |
Pseudomonas putida | |
5.1.1.8 | ZnCl2 | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.1.8 | 22 | - |
hydroxy-L-Pro | - |
Pseudomonas putida | |
5.1.1.8 | 53 | - |
allohydroxy-L-Pro | - |
Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.1.8 | 17930 | - |
low speed sedimentation without reaching equilibrium | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.8 | Hydroxy-L-Pro | Pseudomonas putida | trans-hydroxy-L-Pro or cis-hydroxy-L-Pro, initial reaction of the pathway of hydroxyproline metabolism | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.1.8 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.1.8 | - |
Pseudomonas putida |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
5.1.1.8 | trans-4-Hydroxy-L-proline = cis-4-hydroxy-D-proline | one hydrogen in the substrate is labilized during the reaction | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.1.1.8 | 100 | - |
- |
Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
5.1.1.8 | -15°C, concentrated purified enzyme is stable for at least several weeks | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.8 | Allohydroxy-D-Pro | - |
Pseudomonas putida | Allohydroxy-L-Pro | - |
? | |
5.1.1.8 | D-Ala | at 1-2% of the activity relative to allo-D-hydroxy-proline epimerization | Pseudomonas putida | L-Ala | - |
? | |
5.1.1.8 | Hydroxy-L-Pro | trans-hydroxy-L-Pro or cis-hydroxy-L-Pro, initial reaction of the pathway of hydroxyproline metabolism | Pseudomonas putida | ? | - |
? | |
5.1.1.8 | trans-4-hydroxy-L-Pro | - |
Pseudomonas putida | cis-4-hydroxy-D-Pro | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.1.8 | 7.4 | 8 | allohydroxy-L-proline | Pseudomonas putida |
5.1.1.8 | 8 | - |
hydroxy-L-proline | Pseudomonas putida |