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Literature summary extracted from

  • Gibbs, R.G.; Morris, J.G.
    Glycine-Glyoxylate metabolism: beta-hydroxyaspartate pathway (Micrococcus denitrificans) (1970), Methods Enzymol., 17A, 981-992.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.20 EDTA
-
 Paracoccus denitrificans
4.3.1.20 hydroxylamine
-
 Paracoccus denitrificans
4.3.1.20 Maleate competitive Paracoccus denitrificans
4.3.1.20 p-chloromercuribenzoate
-
 Paracoccus denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.20 0.43
-
 erythro-L-beta-hydroxyaspartate
-
 Paracoccus denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.20 Ca2+ required for activity Paracoccus denitrificans
4.3.1.20 Mg2+ required for activity Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.20 Erythro-3-hydroxyaspartate Paracoccus denitrificans glycine-glycolate metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.20 Paracoccus denitrificans
-
 ATCC 8944
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.20 partial Paracoccus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.20 7.15
-
-
 Paracoccus denitrificans

Storage Stability

EC Number Storage Stability Organism
4.3.1.20 -15°C, with 0.01M Tris-HCl buffer, pH 8, 1 mM MgCl2 and 0.01 mM pyridoxal 5'-phosphate, 50% loss of activity in 60 days Paracoccus denitrificans
4.3.1.20 2°C, with 0.01M Tris-HCl buffer, pH 8, 1 mM MgCl2 and 0.01 mM pyridoxal 5'-phosphate, 50% loss of activity in 10 days Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.20 Erythro-3-hydroxyaspartate glycine-glycolate metabolism Paracoccus denitrificans ?
-
 ?
4.3.1.20 Erythro-DL-beta-hydroxyaspartate
-
 Paracoccus denitrificans Oxaloacetate + NH3
-
 ?
4.3.1.20 erythro-L-beta-hydroxyaspartate
-
 Paracoccus denitrificans oxaloacetate + NH3
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.20 9.5
-
-
 Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.20 pyridoxal 5'-phosphate required for activity Paracoccus denitrificans