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Literature summary extracted from

  • Colby, S.M.; Alonso, W.R.; Katahira, E.J.; McGarvey, D.J.; Croteau, R.
    4S-limonene synthase from the oil glands of spearmint (Mentha spicata): cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase (1993), J. Biol. Chem., 268, 23016-23024.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.16 expressed in Escherichia coli Mentha sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.16 Mg2+ cofactor Mentha sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.16 56000
-
 SDS-PAGE, gel filtration Mentha sp.
4.2.3.16 59800
-
 calculated from cDNA sequence Mentha sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.16 geranyl diphosphate Mentha sp.
-
 (-)-(4S)-limonene + diphosphate small amounts of (-)-alpha-pinene, (-)-beta-pinene and myrcene as side products ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.16 Mentha sp.
-
 M. spicata
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.16
-
 Mentha sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.16 leaf glandular trichome secretory cells Mentha sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.16 geranyl diphosphate
-
 Mentha sp. (-)-(4S)-limonene + diphosphate small amounts of (-)-alpha-pinene, (-)-beta-pinene and myrcene as side products ?