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Literature summary extracted from

  • Bohlmann, J.; Steele C.L.; Croteau, R.
    Monoterpene synthases from grand fir (Abies grandis): cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase (1997), J. Biol. Chem., 272, 21784-21792.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.119 additional information activity increases upon stem wounding Abies grandis
4.2.3.120 additional information activity increases upon stem wounding Abies grandis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.15 expressed in Escherichia coli Abies grandis
4.2.3.16 expressed in Escherichia coli Abies grandis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.15 K+ required, optimum concentration of 0.5 M Abies grandis
4.2.3.15 Mg2+ ineffective Abies grandis
4.2.3.15 Mn2+ required Abies grandis
4.2.3.16 K+ required, optimum concentration of 0.5 M Abies grandis
4.2.3.16 Mg2+ ineffective Abies grandis
4.2.3.16 Mn2+ required Abies grandis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.15 72500
-
 calculated MW of recombinant protein expressed in Escherichia coli Abies grandis
4.2.3.16 73500
-
 calculated MW of recombinant protein expressed in Escherichia coli Abies grandis
4.2.3.119 71500
-
 x * 71500, calculated Abies grandis
4.2.3.120 71500
-
 x * 71500, calculated Abies grandis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.15 geranyl diphosphate Abies grandis induced by stem wounding, enhances resistance to insects myrcene + diphosphate
-
 ?
4.2.3.16 geranyl diphosphate Abies grandis induced by stem wounding, enhances resistance to insects (-)-(4S)-limonene + diphosphate small amounts of (-)-alpha-pinene, (-)-beta-pinene and beta-phellandrene as side products ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.15 Abies grandis O24474 grand fir
-
4.2.3.16 Abies grandis O22340 grand fir
-
4.2.3.119 Abies grandis O24475
-
-
4.2.3.120 Abies grandis O24475
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.3.119 proteolytic modification sequence contains a putative N-terminal plastid targeting signal Abies grandis
4.2.3.120 proteolytic modification sequence contains a putative N-terminal plastid targeting signal Abies grandis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.15 stem
-
 Abies grandis
-
4.2.3.16 stem
-
 Abies grandis
-
4.2.3.119 stem activity increases upon stem wounding Abies grandis
-
4.2.3.120 stem activity increases upon stem wounding Abies grandis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.15 geranyl diphosphate
-
 Abies grandis myrcene + diphosphate
-
 ?
4.2.3.15 geranyl diphosphate induced by stem wounding, enhances resistance to insects Abies grandis myrcene + diphosphate
-
 ?
4.2.3.16 geranyl diphosphate
-
 Abies grandis (-)-(4S)-limonene + diphosphate small amounts of (-)-alpha-pinene, (-)-beta-pinene and beta-phellandrene as side products ?
4.2.3.16 geranyl diphosphate induced by stem wounding, enhances resistance to insects Abies grandis (-)-(4S)-limonene + diphosphate small amounts of (-)-alpha-pinene, (-)-beta-pinene and beta-phellandrene as side products ?
4.2.3.119 geranyl diphosphate
-
 Abies grandis (-)-alpha-pinene + diphosphate products are (-)-alpha-pinene and (-)-beta pinene ?
4.2.3.120 geranyl diphosphate
-
 Abies grandis (-)-beta-pinene + diphosphate products are (-)-alpha-pinene and (-)-beta pinene ?

Subunits

EC Number Subunits Comment Organism
4.2.3.119 ? x * 71500, calculated Abies grandis
4.2.3.120 ? x * 71500, calculated Abies grandis