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Literature summary extracted from

  • Nandi, D.L.; Shemin, D.
    delta-Aminolevulinic acid dehydratase of Rhodopseudomonas capsulata (1973), Arch. Biochem. Biophys., 158, 305-311.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.24 2-mercaptoethanol required at very low concentrations of substrate Rhodobacter capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.24 EDTA no inhibition Rhodobacter capsulatus
4.2.1.24 iodoacetamide
-
 Rhodobacter capsulatus
4.2.1.24 additional information insensitive to inhibition by hemin and protoporphyrin Rhodobacter capsulatus
4.2.1.24 NaCN in presence of the substrate Rhodobacter capsulatus
4.2.1.24 NEM
-
 Rhodobacter capsulatus
4.2.1.24 PCMB
-
 Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.24 0.7
-
 5-aminolevulinate in presence of 2-mercaptoethanol Rhodobacter capsulatus
4.2.1.24 2
-
 5-aminolevulinate in absence of 2-mercaptoethanol Rhodobacter capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.24 additional information does not require metallic cations for activation Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.24 260000
-
 sucrose density gradient centrifugation Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.24 Rhodobacter capsulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.24
-
 Rhodobacter capsulatus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.24 2 5-aminolevulinate = porphobilinogen + 2 H2O substrate is covalently bound through a Schiff base Rhodobacter capsulatus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.24 1.62
-
-
 Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.24 5-aminolevulinate + 5-aminolevulinate
-
 Rhodobacter capsulatus porphobilinogen + 2 H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.24 8 8.6 in citrate buffer, Tris-maleate buffer, imidazole buffer and Tris buffer Rhodobacter capsulatus