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Literature summary extracted from
- Purification of porphobilinogen synthase from bovine liver (1986), Methods Enzymol., 123, 427-434.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.24 | thiol | 20 mM, essential to maintain the enzyme in its reduced state | Bos taurus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.24 | 5-bromolevulinic acid | - |
Bos taurus | |
| 4.2.1.24 | 5-chlorolevulinic acid | - |
Bos taurus | |
| 4.2.1.24 | 5-iodolevulinic acid | - |
Bos taurus | |
| 4.2.1.24 | DTNB | reversible loss of activity | Bos taurus | |
| 4.2.1.24 | EDTA | - |
Bos taurus | |
| 4.2.1.24 | iodoacetamide | irreversible | Bos taurus | |
| 4.2.1.24 | iodoacetate | irreversible | Bos taurus | |
| 4.2.1.24 | Mercury ions | - |
Bos taurus | |
| 4.2.1.24 | Pb2+ | - |
Bos taurus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.24 | Zn2+ | required | Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.24 | 35000 | - |
8 * 35000, SDS-PAGE | Bos taurus |
| 4.2.1.24 | 280000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.24 | 5-aminolevulinate | Bos taurus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.24 | Bos taurus | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 4.2.1.24 | total but reversible loss of activity by O2 | Bos taurus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.24 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.1.24 | liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.24 | 0.63 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.24 | 5-aminolevulinate | - |
Bos taurus | ? | - |
? | |
| 4.2.1.24 | 5-aminolevulinate + 5-aminolevulinate | - |
Bos taurus | porphobilinogen + 2 H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.24 | octamer | 8 * 35000, SDS-PAGE | Bos taurus |
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Release 2023.1 (January 2023)
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