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Literature summary extracted from

  • Kawasaki, H.; Miyoshi, K.; Tonomura, K.
    Purification, crystallization and properties of haloacetate halidohydrolase from Pseudomonas species (1981), Agric. Biol. Chem., 45, 543-544.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.8.1.3
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.3 2
-
 monofluoroacetate
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.3 33000
-
 2 * 33000, SDS-PAGE Pseudomonas sp.
3.8.1.3 42000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.3 monofluoroacetate + H2O Pseudomonas sp.
-
 glycolate + HF
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.3 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.3 H1 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.3 38.4
-
 crystallized Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.3 monofluoroacetate + H2O
-
 Pseudomonas sp. glycolate + HF
-
 ir

Subunits

EC Number Subunits Comment Organism
3.8.1.3 dimer 2 * 33000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.8.1.3 monohaloacetate dehalogenase
-
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.3 50
-
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.3 9
-
-
 Pseudomonas sp.