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Literature summary extracted from

  • Kawasaki, H.; Tone, N.; Tonomura, K.
    Purification and properties of haloacetate halidohydrolase specified by plasmid from Moraxella sp. strain B (1981), Agric. Biol. Chem., 45, 35-42.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.3 AgCl2
-
 Moraxella sp.
3.8.1.3 HgCl2
-
 Moraxella sp.
3.8.1.3 N-ethylmaleimide
-
 Moraxella sp.
3.8.1.3 N-ethylmaleimide
-
 Pseudomonas sp.
3.8.1.3 p-chloromercuribenzoate
-
 Moraxella sp.
3.8.1.3 p-chloromercuriphenylsulfonate
-
 Moraxella sp.
3.8.1.3 p-chloromercuriphenylsulfonate
-
 Pseudomonas sp.
3.8.1.3 thiol reagents
-
 Moraxella sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.3 1.1
-
 monoiodoacetate
-
 Pseudomonas sp.
3.8.1.3 1.1
-
 monoiodoacetate
-
 Moraxella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.3 43000
-
 gel filtration Moraxella sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.3 monobromoacetate + H2O Pseudomonas sp. H2 glycolate + HBr
-
 ir
3.8.1.3 monobromoacetate + H2O Moraxella sp. H2 glycolate + HBr
-
 ir
3.8.1.3 monochloroacetate + H2O Pseudomonas sp. H1,H2 glycolate + HCl
-
 ir
3.8.1.3 monochloroacetate + H2O Moraxella sp. H1,H2 glycolate + HCl
-
 ir
3.8.1.3 monofluoroacetate + H2O Pseudomonas sp.
-
 glycolate + HF
-
 ?
3.8.1.3 monofluoroacetate + H2O Moraxella sp. H1 glycolate + HF
-
 ir
3.8.1.3 monoiodoacetate + H2O Pseudomonas sp. H2 glycolate + HI
-
 ir
3.8.1.3 monoiodoacetate + H2O Moraxella sp. H2 glycolate + HI
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.3 Moraxella sp.
-
-
-
3.8.1.3 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.3 H2 Moraxella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.3 monobromoacetate + H2O H2 Pseudomonas sp. glycolate + HBr
-
 ir
3.8.1.3 monobromoacetate + H2O H2 Moraxella sp. glycolate + HBr
-
 ir
3.8.1.3 monochloroacetate + H2O H1,H2 Pseudomonas sp. glycolate + HCl
-
 ir
3.8.1.3 monochloroacetate + H2O H1,H2 Moraxella sp. glycolate + HCl
-
 ir
3.8.1.3 monofluoroacetate + H2O
-
 Pseudomonas sp. glycolate + HF
-
 ?
3.8.1.3 monofluoroacetate + H2O H1 Moraxella sp. glycolate + HF
-
 ir
3.8.1.3 monoiodoacetate + H2O H2 Pseudomonas sp. glycolate + HI
-
 ir
3.8.1.3 monoiodoacetate + H2O H2 Moraxella sp. glycolate + HI
-
 ir

Subunits

EC Number Subunits Comment Organism
3.8.1.3 dimer
-
 Pseudomonas sp.
3.8.1.3 dimer 2 * 26000-27000 Moraxella sp.

Synonyms

EC Number Synonyms Comment Organism
3.8.1.3 monohaloacetate dehalogenase
-
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.3 50
-
-
 Pseudomonas sp.
3.8.1.3 50
-
-
 Moraxella sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.8.1.3 30 60
-
 Pseudomonas sp.
3.8.1.3 30 60
-
 Moraxella sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.8.1.3 20 60
-
 Pseudomonas sp.
3.8.1.3 20 60 rapid denaturation over 70°C Moraxella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.3 9.5
-
-
 Pseudomonas sp.
3.8.1.3 9.5
-
-
 Moraxella sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.8.1.3 8 11
-
 Moraxella sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.8.1.3 5 10
-
 Moraxella sp.