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Literature summary extracted from
- Cloning and characterization of oah, the gene encoding oxaloacetate hydrolase in Aspergillus niger (2000), Mol. Gen. Genet., 263, 281-286.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.7.1.1 | cloned by inverse PCR into Escherichia coli | Aspergillus niger |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.7.1.1 | 0.004 | - |
Mn2+ | - |
Aspergillus niger |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.1 | 39000 | - |
10-12 * 39000, SDS-PAGE | Aspergillus niger |
| 3.7.1.1 | 360000 | 440000 | two major bands, native PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.1 | oxaloacetate + H2O | Aspergillus niger | could be involved in pathogenesis and keeping pH low in vicinity of the fungus | oxalate + acetate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.7.1.1 | Aspergillus niger | - |
inducible enzyme, expressed only at pH values higher than 4.0 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.7.1.1 | Phenyl Sepharose, Q-Sepharose | Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.1 | 56 | - |
- |
Aspergillus niger |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.7.1.1 | 4°C, 50 mM Tris-HCL, pH 7.5, 2 mM MnCl2, 20 mM DTT 5% sucrose, 7 days | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.1 | oxaloacetate + H2O | could be involved in pathogenesis and keeping pH low in vicinity of the fungus | Aspergillus niger | oxalate + acetate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.7.1.1 | oligomer | 10-12 * 39000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.1 | 45 | - |
- |
Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.1 | 7 | - |
- |
Aspergillus niger |
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Release 2023.1 (January 2023)
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