BRENDA - Enzyme Database

Purification and properties of C 55 -isoprenylpyrophosphate phosphatase from Micrococcus lysodeikticus

Goldman, R.; Strominger, J.L.; J. Biol. Chem. 247, 5116-5122 (1972)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.27
ethanol
slight activation at concentration of 4 M
Micrococcus luteus
3.6.1.27
methanol
slight activation at concentration of 4 M
Micrococcus luteus
3.6.1.27
additional information
significant rise in activity at high ionic strength
Micrococcus luteus
3.6.1.27
n-Propanol
activating at concentrations below 1.5 M
Micrococcus luteus
3.6.1.27
Triton X-100
-
Micrococcus luteus
3.6.1.27
Triton X-114
-
Micrococcus luteus
General Stability
EC Number
General Stability
Organism
3.6.1.27
ascorbic acid stabilizes
Micrococcus luteus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.27
DTNB
slight inhibition
Micrococcus luteus
3.6.1.27
n-butanol
-
Micrococcus luteus
3.6.1.27
n-Propanol
inhibitory at concentrations above 1.5 M
Micrococcus luteus
3.6.1.27
p-chloromercuriphenylsulfonic acid
-
Micrococcus luteus
3.6.1.27
tetrahydrofuran
inhibitory at concentrations above 2 M
Micrococcus luteus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.27
0.4
-
undecaprenyl diphosphate
-
Micrococcus luteus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.27
membrane
-
Micrococcus luteus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.27
additional information
no requirement for added metal ions
Micrococcus luteus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.6.1.27
undecaprenyl diphosphate + H2O
Micrococcus luteus
involved in cyclic peptidoglycan pathway
undecaprenyl phosphate + phosphate
-
Micrococcus luteus
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.6.1.27
Micrococcus luteus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.6.1.27
partial
Micrococcus luteus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.6.1.27
undecaprenyl diphosphate + H2O
-
210607
Micrococcus luteus
undecaprenyl phosphate + phosphate
-
210607
Micrococcus luteus
?
3.6.1.27
undecaprenyl diphosphate + H2O
involved in cyclic peptidoglycan pathway
210607
Micrococcus luteus
undecaprenyl phosphate + phosphate
-
210607
Micrococcus luteus
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.6.1.27
-20
-
significant loss of activity by freezing and thawing one time
Micrococcus luteus
3.6.1.27
4
-
30-40% loss of activity within 1 week
Micrococcus luteus
3.6.1.27
25
-
solubilized enzyme stable for 30 min below 25°C, loss of activity with higher temperatures
Micrococcus luteus
3.6.1.27
40
-
particular enzyme stable for 30 min below 40°C, loss of activity with higher temperatures
Micrococcus luteus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.27
7.3
7.5
-
Micrococcus luteus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.27
5
9
-
Micrococcus luteus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.27
ethanol
slight activation at concentration of 4 M
Micrococcus luteus
3.6.1.27
methanol
slight activation at concentration of 4 M
Micrococcus luteus
3.6.1.27
additional information
significant rise in activity at high ionic strength
Micrococcus luteus
3.6.1.27
n-Propanol
activating at concentrations below 1.5 M
Micrococcus luteus
3.6.1.27
Triton X-100
-
Micrococcus luteus
3.6.1.27
Triton X-114
-
Micrococcus luteus
General Stability (protein specific)
EC Number
General Stability
Organism
3.6.1.27
ascorbic acid stabilizes
Micrococcus luteus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.27
DTNB
slight inhibition
Micrococcus luteus
3.6.1.27
n-butanol
-
Micrococcus luteus
3.6.1.27
n-Propanol
inhibitory at concentrations above 1.5 M
Micrococcus luteus
3.6.1.27
p-chloromercuriphenylsulfonic acid
-
Micrococcus luteus
3.6.1.27
tetrahydrofuran
inhibitory at concentrations above 2 M
Micrococcus luteus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.27
0.4
-
undecaprenyl diphosphate
-
Micrococcus luteus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.27
membrane
-
Micrococcus luteus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.27
additional information
no requirement for added metal ions
Micrococcus luteus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.6.1.27
undecaprenyl diphosphate + H2O
Micrococcus luteus
involved in cyclic peptidoglycan pathway
undecaprenyl phosphate + phosphate
-
Micrococcus luteus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.27
partial
Micrococcus luteus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.6.1.27
undecaprenyl diphosphate + H2O
-
210607
Micrococcus luteus
undecaprenyl phosphate + phosphate
-
210607
Micrococcus luteus
?
3.6.1.27
undecaprenyl diphosphate + H2O
involved in cyclic peptidoglycan pathway
210607
Micrococcus luteus
undecaprenyl phosphate + phosphate
-
210607
Micrococcus luteus
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.6.1.27
-20
-
significant loss of activity by freezing and thawing one time
Micrococcus luteus
3.6.1.27
4
-
30-40% loss of activity within 1 week
Micrococcus luteus
3.6.1.27
25
-
solubilized enzyme stable for 30 min below 25°C, loss of activity with higher temperatures
Micrococcus luteus
3.6.1.27
40
-
particular enzyme stable for 30 min below 40°C, loss of activity with higher temperatures
Micrococcus luteus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.27
7.3
7.5
-
Micrococcus luteus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.27
5
9
-
Micrococcus luteus