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Literature summary extracted from

  • Goldman, R.; Strominger, J.L.
    Purification and properties of C 55 -isoprenylpyrophosphate phosphatase from Micrococcus lysodeikticus (1972), J. Biol. Chem., 247, 5116-5122.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.27 ethanol slight activation at concentration of 4 M Micrococcus luteus
3.6.1.27 methanol slight activation at concentration of 4 M Micrococcus luteus
3.6.1.27 additional information significant rise in activity at high ionic strength Micrococcus luteus
3.6.1.27 n-Propanol activating at concentrations below 1.5 M Micrococcus luteus
3.6.1.27 Triton X-100
-
Micrococcus luteus
3.6.1.27 Triton X-114
-
Micrococcus luteus

General Stability

EC Number General Stability Organism
3.6.1.27 ascorbic acid stabilizes Micrococcus luteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.27 DTNB slight inhibition Micrococcus luteus
3.6.1.27 n-butanol
-
Micrococcus luteus
3.6.1.27 n-Propanol inhibitory at concentrations above 1.5 M Micrococcus luteus
3.6.1.27 p-chloromercuriphenylsulfonic acid
-
Micrococcus luteus
3.6.1.27 tetrahydrofuran inhibitory at concentrations above 2 M Micrococcus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.27 0.4
-
undecaprenyl diphosphate
-
Micrococcus luteus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.27 membrane
-
Micrococcus luteus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.27 additional information no requirement for added metal ions Micrococcus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.27 undecaprenyl diphosphate + H2O Micrococcus luteus involved in cyclic peptidoglycan pathway undecaprenyl phosphate + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.27 Micrococcus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.27 partial Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.27 undecaprenyl diphosphate + H2O
-
Micrococcus luteus undecaprenyl phosphate + phosphate
-
?
3.6.1.27 undecaprenyl diphosphate + H2O involved in cyclic peptidoglycan pathway Micrococcus luteus undecaprenyl phosphate + phosphate
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.27 -20
-
significant loss of activity by freezing and thawing one time Micrococcus luteus
3.6.1.27 4
-
30-40% loss of activity within 1 week Micrococcus luteus
3.6.1.27 25
-
solubilized enzyme stable for 30 min below 25°C, loss of activity with higher temperatures Micrococcus luteus
3.6.1.27 40
-
particular enzyme stable for 30 min below 40°C, loss of activity with higher temperatures Micrococcus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.27 7.3 7.5
-
Micrococcus luteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.27 5 9
-
Micrococcus luteus