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Literature summary extracted from

  • Endow, S.A.; Kang, S.J.; Satterwhite, L.L.; Rose, M.D.; Skeen, V.P.; Salmon, E.D.
    Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends (1994), EMBO J., 13, 2708-2713.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.4 DNA sequence analysis of KAR3, Kar3 cloned and expressed in Escherichia coli BL21(DE3)pLysS as fusion protein with glutathione S-transferase, GST-Kar3 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n Drosophila melanogaster
-
 ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n Saccharomyces cerevisiae
-
 ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n Arabidopsis sp.
-
 ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.4 Arabidopsis sp.
-
-
-
5.6.1.4 Drosophila melanogaster
-
-
-
5.6.1.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.4 fusion protein GST-Kar3 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Drosophila melanogaster
a
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Saccharomyces cerevisiae
a
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Arabidopsis sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila melanogaster ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Saccharomyces cerevisiae ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Arabidopsis sp. ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.4 Kar3
-
 Saccharomyces cerevisiae
5.6.1.4 ncd
-
 Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.6.1.4 0.0008
-
 ATP
-
 Saccharomyces cerevisiae