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Literature summary extracted from
- Cloning of Chlamydomonas p60 katanin and localization to the site of outer doublet severing during deflagellation (1999), Cell Motil. Cytoskeleton, 43, 221-231.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.6.1.1 | - |
Strongylocentrotus purpuratus |
| 5.6.1.1 | cloning of Cp60 katanin cDNA, expressed in Escherichia coli BL21(DE3) | Chlamydomonas reinhardtii |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.6.1.1 | 52000 | - |
proteolytic product of the p60 subunit, SDS-PAGE | Chlamydomonas reinhardtii |
| 5.6.1.1 | 60000 | - |
1 * 80000 + 1 * 60000, SDS-PAGE | Chlamydomonas reinhardtii |
| 5.6.1.1 | 60000 | - |
1 * 80000 + 1 * 60000, SDS-PAGE | Strongylocentrotus purpuratus |
| 5.6.1.1 | 60100 | - |
theoretical value, calculated from amino acid sequence | Chlamydomonas reinhardtii |
| 5.6.1.1 | 62000 | - |
recombinant Cp60 | Chlamydomonas reinhardtii |
| 5.6.1.1 | 80000 | - |
1 * 80000 + 1 * 60000, SDS-PAGE | Chlamydomonas reinhardtii |
| 5.6.1.1 | 80000 | - |
1 * 80000 + 1 * 60000, SDS-PAGE | Strongylocentrotus purpuratus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.1.1 | ATP + H2O | Chlamydomonas reinhardtii | - |
ADP + phosphate | - |
? |  |
| 5.6.1.1 | ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? | |
| 5.6.1.1 | ATP + H2O | Xenopus laevis | - |
ADP + phosphate | - |
? | |
| 5.6.1.1 | ATP + H2O | Strongylocentrotus purpuratus | - |
ADP + phosphate | - |
? | |
| 5.6.1.1 | GTP + H2O | Chlamydomonas reinhardtii | - |
GDP + phosphate | - |
? | |
| 5.6.1.1 | GTP + H2O | Homo sapiens | - |
GDP + phosphate | - |
? | |
| 5.6.1.1 | GTP + H2O | Xenopus laevis | - |
GDP + phosphate | - |
? | |
| 5.6.1.1 | GTP + H2O | Strongylocentrotus purpuratus | - |
GDP + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.1.1 | Chlamydomonas reinhardtii | - |
- |
- |
| 5.6.1.1 | Homo sapiens | - |
human | - |
| 5.6.1.1 | Homo sapiens | - |
human cell lines MSU1.1 and HeLa | - |
| 5.6.1.1 | Strongylocentrotus purpuratus | - |
sea urchin | - |
| 5.6.1.1 | Xenopus laevis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.6.1.1 | - |
Strongylocentrotus purpuratus |
| 5.6.1.1 | p60, 60 kDa catalytic subunit | Chlamydomonas reinhardtii |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 5.6.1.1 | n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome | Chlamydomonas reinhardtii | |
| 5.6.1.1 | n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome | Homo sapiens | |
| 5.6.1.1 | n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome | Xenopus laevis | |
| 5.6.1.1 | n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers | another member of the AAA-ATPase family, active in splitting microtubules into tubulin dimers in the centrosome | Strongylocentrotus purpuratus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.6.1.1 | oocyte | - |
Xenopus laevis | - |
| 5.6.1.1 | oocyte | - |
Strongylocentrotus purpuratus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.1.1 | ATP + H2O | - |
Chlamydomonas reinhardtii | ADP + phosphate | - |
? | |
| 5.6.1.1 | ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
| 5.6.1.1 | ATP + H2O | - |
Xenopus laevis | ADP + phosphate | - |
? | |
| 5.6.1.1 | ATP + H2O | - |
Strongylocentrotus purpuratus | ADP + phosphate | - |
? | |
| 5.6.1.1 | GTP + H2O | - |
Chlamydomonas reinhardtii | GDP + phosphate | - |
? | |
| 5.6.1.1 | GTP + H2O | - |
Homo sapiens | GDP + phosphate | - |
? | |
| 5.6.1.1 | GTP + H2O | - |
Xenopus laevis | GDP + phosphate | - |
? | |
| 5.6.1.1 | GTP + H2O | - |
Strongylocentrotus purpuratus | GDP + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.6.1.1 | dimer | - |
Xenopus laevis |
| 5.6.1.1 | dimer | 1 * 80000 + 1 * 60000, SDS-PAGE | Chlamydomonas reinhardtii |
| 5.6.1.1 | dimer | 1 * 80000 + 1 * 60000, SDS-PAGE | Strongylocentrotus purpuratus |
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