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Literature summary extracted from

  • Abrams, A.; Smith, J.B.
    Bacterial membrane ATPase (1974), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 10, 395-429.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.2.2 ADP
-
 Enterococcus faecalis
7.1.2.2 Ca2+
-
 Escherichia coli
7.1.2.2 CN-
-
 Geobacillus stearothermophilus
7.1.2.2 guanidine
-
 Enterococcus faecalis
7.1.2.2 Hg2+
-
 Escherichia coli
7.1.2.2 I2
-
 Geobacillus stearothermophilus
7.1.2.2 Mg2+ Ca2+-activated enzyme Micrococcus luteus
7.1.2.2 N,N'-dicyclohexylcarbodiimide
-
 Enterococcus faecalis
7.1.2.2 N,N'-dicyclohexylcarbodiimide
-
 Escherichia coli
7.1.2.2 N3-
-
 Escherichia coli
7.1.2.2 N3-
-
 Geobacillus stearothermophilus
7.1.2.2 N3-
-
 Micrococcus luteus
7.1.2.2 N3-
-
 Priestia megaterium
7.1.2.2 phosphate
-
 Enterococcus faecalis
7.1.2.2 phosphate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.2.2 0.6
-
 ATP
-
 Escherichia coli
7.1.2.2 0.9
-
 ATP strain NRLL B939 Priestia megaterium
7.1.2.2 1
-
 ATP strain KM Priestia megaterium
7.1.2.2 2.5
-
 ATP
-
 Enterococcus faecalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.2.2 membrane
-
 Alcaligenes faecalis 16020
-
7.1.2.2 membrane
-
 Escherichia coli 16020
-
7.1.2.2 membrane
-
 Geobacillus stearothermophilus 16020
-
7.1.2.2 membrane
-
 Cereibacter sphaeroides 16020
-
7.1.2.2 membrane
-
 Priestia megaterium 16020
-
7.1.2.2 membrane
-
 Enterococcus faecalis 16020
-
7.1.2.2 membrane exclusively internal location Micrococcus luteus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.2.2 Ca2+
-
 Geobacillus stearothermophilus
7.1.2.2 Ca2+ activates Alcaligenes faecalis
7.1.2.2 Ca2+ activates Escherichia coli
7.1.2.2 Ca2+ activates Cereibacter sphaeroides
7.1.2.2 Ca2+ activates Priestia megaterium
7.1.2.2 Ca2+ activates Enterococcus faecalis
7.1.2.2 Ca2+ activates Micrococcus luteus
7.1.2.2 Cd2+ activates Priestia megaterium
7.1.2.2 Co2+ activates Escherichia coli
7.1.2.2 Co2+ activates Geobacillus stearothermophilus
7.1.2.2 Mg2+ activates Alcaligenes faecalis
7.1.2.2 Mg2+ activates Escherichia coli
7.1.2.2 Mg2+ activates Geobacillus stearothermophilus
7.1.2.2 Mg2+ activates Cereibacter sphaeroides
7.1.2.2 Mg2+ activates Priestia megaterium
7.1.2.2 Mg2+ activates Enterococcus faecalis
7.1.2.2 Mg2+ activates Micrococcus luteus
7.1.2.2 Mg2+ absolute requirement for Mg2+ or Mn2+ Enterococcus faecalis
7.1.2.2 Mn2+ activates Escherichia coli
7.1.2.2 Mn2+ activates Geobacillus stearothermophilus
7.1.2.2 Mn2+ activates Enterococcus faecalis
7.1.2.2 Mn2+ absolute requirement for Mg2+ or Mn2+ Enterococcus faecalis
7.1.2.2 Zn2+ activates Escherichia coli
7.1.2.2 Zn2+ activates Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.2.2 47000
-
 alpha6,beta6, 6 * 47000 + 6 * 51000, SDS-PAGE Enterococcus faecalis
7.1.2.2 51000
-
 alpha6,beta6, 6 * 47000 + 6 * 51000, SDS-PAGE Enterococcus faecalis
7.1.2.2 280000
-
 equilibrium sedimentation Geobacillus stearothermophilus
7.1.2.2 300000
-
 gel filtration Alcaligenes faecalis
7.1.2.2 300000
-
 gel filtration Cereibacter sphaeroides
7.1.2.2 379000
-
 strain KM, sedimentation equilibrium centrifugation Priestia megaterium
7.1.2.2 385000
-
 sedimentation equilibrium analysis Enterococcus faecalis
7.1.2.2 400000
-
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
7.1.2.2 Alcaligenes faecalis
-
-
-
7.1.2.2 Cereibacter sphaeroides
-
-
-
7.1.2.2 Enterococcus faecalis
-
-
-
7.1.2.2 Escherichia coli
-
-
-
7.1.2.2 Geobacillus stearothermophilus
-
-
-
7.1.2.2 Micrococcus luteus
-
-
-
7.1.2.2 Priestia megaterium
-
 and strain KM
-
7.1.2.2 Priestia megaterium NRLL B939
-
 and strain KM
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.2.2
-
 Priestia megaterium
7.1.2.2
-
 Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.2.2 2'-deoxy-ATP + H2O
-
 Enterococcus faecalis 2'-deoxy-ADP + phosphate + H+/out
-
 ?
7.1.2.2 ATP + H2O + H+/in
-
 Alcaligenes faecalis ADP + phosphate + H+/out
-
 ?
7.1.2.2 ATP + H2O + H+/in
-
 Escherichia coli ADP + phosphate + H+/out
-
 ?
7.1.2.2 ATP + H2O + H+/in
-
 Geobacillus stearothermophilus ADP + phosphate + H+/out
-
 ?
7.1.2.2 ATP + H2O + H+/in
-
 Cereibacter sphaeroides ADP + phosphate + H+/out
-
 ?
7.1.2.2 ATP + H2O + H+/in
-
 Priestia megaterium ADP + phosphate + H+/out
-
 ?
7.1.2.2 ATP + H2O + H+/in
-
 Enterococcus faecalis ADP + phosphate + H+/out
-
 ?
7.1.2.2 ATP + H2O + H+/in
-
 Micrococcus luteus ADP + phosphate + H+/out
-
 ?
7.1.2.2 ATP + H2O + H+/in
-
 Priestia megaterium NRLL B939 ADP + phosphate + H+/out
-
 ?
7.1.2.2 CTP + H2O + H+/in poorly hydrolyzed Enterococcus faecalis CDP + phosphate + H+/out
-
 ?
7.1.2.2 GTP + H2O + H+/in
-
 Enterococcus faecalis GDP + phosphate + H+/out
-
 ?
7.1.2.2 ITP + H2O + H+/in
-
 Enterococcus faecalis IDP + phosphate + H+/out
-
 ?
7.1.2.2 UTP + H2O + H+/in poorly hydrolyzed Enterococcus faecalis UDP + phosphate + H+/out
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.2.2 dodecamer alpha6,beta6, 6 * 47000 + 6 * 51000, SDS-PAGE Enterococcus faecalis
7.1.2.2 More size of major subunits Escherichia coli
7.1.2.2 More size of major subunits Priestia megaterium
7.1.2.2 More size of major subunits Enterococcus faecalis
7.1.2.2 More size of major subunits Micrococcus luteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
7.1.2.2 additional information
-
 no cold lability Geobacillus stearothermophilus
7.1.2.2 additional information
-
 cold lability Escherichia coli
7.1.2.2 additional information
-
 cold lability Priestia megaterium
7.1.2.2 additional information
-
 cold lability Enterococcus faecalis
7.1.2.2 additional information
-
 cold lability Micrococcus luteus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.2.2 217
-
 ATP
-
 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.2.2 6 8
-
 Enterococcus faecalis
7.1.2.2 7.5
-
-
 Micrococcus luteus
7.1.2.2 8 8.5
-
 Geobacillus stearothermophilus
7.1.2.2 8.1
-
-
 Cereibacter sphaeroides
7.1.2.2 9 9.5
-
 Escherichia coli