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Literature summary extracted from

  • Guranowski, A.; Starzynska, E.; Rataj-Guranowska, M.; Sillero, M.A.G.
    Purification of apyrase from yellow lupin cotyledons after extraction with perchloric acid (1991), Protein Expr. Purif., 2, 235-239.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 0.02
-
 ATP
-
 Lupinus luteus
3.6.1.5 0.02
-
 ADP
-
 Lupinus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+ either Mg2+ or Ca2+ required for activity Lupinus luteus
3.6.1.5 Mg2+ either Mg2+ or Ca2+ required for activity Lupinus luteus
3.6.1.5 Mn2+ lower activation than Ca2+, Mg2+ Lupinus luteus
3.6.1.5 Zn2+ lower activation than Ca2+, Mg2+ Lupinus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.5 51000
-
 SDS-PAGE Lupinus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.5 ADP + H2O Lupinus luteus
-
 AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O Lupinus luteus
-
 AMP + 2 phosphate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.6.1.5 perchloric acid enzyme remains active Lupinus luteus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Lupinus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.5
-
 Lupinus luteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.5 cotyledon seedling cotyledons Lupinus luteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.5 98
-
-
 Lupinus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 adenosine 5'-tetraphosphate + H2O
-
 Lupinus luteus adenosine 5'-monophosphate + phosphate
-
 ?
3.6.1.5 ADP + H2O
-
 Lupinus luteus AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O
-
 Lupinus luteus AMP + 2 phosphate
-
 ?
3.6.1.5 CDP + H2O
-
 Lupinus luteus CMP + phosphate
-
 ?
3.6.1.5 CTP + 2 H2O
-
 Lupinus luteus CMP + 2 phosphate
-
 ?
3.6.1.5 dATP + 2 H2O
-
 Lupinus luteus dAMP + 2 phosphate
-
 ?
3.6.1.5 GDP + H2O
-
 Lupinus luteus GMP + phosphate
-
 ?
3.6.1.5 GTP + 2 H2O
-
 Lupinus luteus GMP + 2 phosphate
-
 ?
3.6.1.5 UDP + H2O
-
 Lupinus luteus UMP + phosphate
-
 ?
3.6.1.5 UTP + 2 H2O
-
 Lupinus luteus UMP + 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.5 monomer 1* 51000 Lupinus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.5 6 8
-
 Lupinus luteus