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Literature summary extracted from

  • Shlomai, J.; Kornberg, A.
    Deoxyuridine triphosphatase of Escherichia coli. Purification, properties, and use as a reagent to reduce uracil incorporation into DNA (1978), J. Biol. Chem., 253, 3305-3312.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.6.1.23 synthesis can reduce uracil incorporation into DNA Escherichia coli

General Stability

EC Number General Stability Organism
3.6.1.23 stable to freezing and thawing Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 EDTA 2 mM, complete inhibition Escherichia coli
3.6.1.23 Mg2+ complete inhibition by extensive dialysis against 0.0125 M MgCl2 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.012
-
 dUTP
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ no requirement for Mg2+ Escherichia coli
3.6.1.23 Zn2+ appears to be essential for activity Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.23 16000
-
 4 * 16000, SDS-PAGE Escherichia coli
3.6.1.23 64500
-
 gel filtration, sucrose density gradient centrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.6.1.23 -70°C, stable for at least 5 months Escherichia coli
3.6.1.23 0°C, stable for at least 3 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Escherichia coli dUMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 tetramer 4 * 16000, SDS-PAGE Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.23 6.1 8 pH 5.0: no activity, pH 6.1-7.5: optimum, pH 8.0: about 60% of maximal activity Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 38.3
-
 dUTP at 30°C Escherichia coli
3.6.1.23 58.3
-
 dUTP at 35°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.23 6.1 7.5
-
 Escherichia coli