EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | 2-mercaptoethanol | 50-500 mM, 25% stimulation | Bacillus subtilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | bacteriophage PBS2-induced inhibitor | seems to form a high MW complex with the Bacillus subtilis dUTPase, the lower MW enzyme form of dUTPase is not inactivated by the protease inhibitor | Bacillus subtilis | |
3.6.1.23 | EDTA | - |
Bacillus subtilis | |
3.6.1.23 | Hg2+ | 10 mM HgCl2, 50% inhibition in absence of mercaptoethanol | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.002 | - |
dUTP | - |
Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Mg2+ | MgCl2 stimulates | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 27000 | - |
low molecular weight enzyme form, gel filtration | Bacillus subtilis |
3.6.1.23 | 48000 | - |
high molecular weight enzyme form, gel filtration | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.23 | Bacillus subtilis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | - |
Bacillus subtilis | dUMP + diphosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 8 | 8.5 | - |
Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 8 | 8.5 | - |
Bacillus subtilis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 6 | 10 | 50% of maximal activity at pH 6 and pH 10 | Bacillus subtilis |