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Literature summary extracted from

  • Gasmi, L.; Cartwright, J.L.; McLennan, A.G.
    Cloning, expression and characterization of YSA1H, a human adenosine 5'-diphosphosugar pyrophosphatase possessing a MutT motif (1999), Biochem. J., 344, 331-337.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.13 expression as a thioredoxin fusion protein in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.13 F- IC50: 0.02 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.13 0.06
-
 ADP-ribose pH 7.5, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.13 Mg2+ optimal activity with 2.5-5 mM Mg2+ or 0.1-0.25 mM Mn2+ Homo sapiens
3.6.1.13 Mn2+ optimal activity with 2.5-5 mM Mg2+ or 0.1-0.25 mM Mn2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.13 24300
-
 2 * 24300, native protein, mass spectrometry Homo sapiens
3.6.1.13 85000
-
 dimeric thioredoxin fusion protein expressed in Escherichia coli, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.13 ADP-ribose + H2O Homo sapiens the function of the enzyme might be to remove free ADP-ribose arising from NAD+ and protein-bound poly- and non-enzymic protein glycation AMP + ribose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.13 Homo sapiens Q9UKK9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.13
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.13 adrenal gland
-
 Homo sapiens
-
3.6.1.13 aorta
-
 Homo sapiens
-
3.6.1.13 bladder
-
 Homo sapiens
-
3.6.1.13 bone marrow low activity Homo sapiens
-
3.6.1.13 brain
-
 Homo sapiens
-
3.6.1.13 caudate nucleus
-
 Homo sapiens
-
3.6.1.13 caudate putamen
-
 Homo sapiens
-
3.6.1.13 cerebellum
-
 Homo sapiens
-
3.6.1.13 cerebral cortex
-
 Homo sapiens
-
3.6.1.13 colon
-
 Homo sapiens
-
3.6.1.13 frontal lobe
-
 Homo sapiens
-
3.6.1.13 heart
-
 Homo sapiens
-
3.6.1.13 hippocampus
-
 Homo sapiens
-
3.6.1.13 kidney high activity Homo sapiens
-
3.6.1.13 leukocyte
-
 Homo sapiens
-
3.6.1.13 liver high activity Homo sapiens
-
3.6.1.13 lung
-
 Homo sapiens
-
3.6.1.13 lymph node low activity Homo sapiens
-
3.6.1.13 mammary gland
-
 Homo sapiens
-
3.6.1.13 medulla oblongata
-
 Homo sapiens
-
3.6.1.13 nucleus accumbens
-
 Homo sapiens
-
3.6.1.13 occipital lobe
-
 Homo sapiens
-
3.6.1.13 ovary
-
 Homo sapiens
-
3.6.1.13 pancreas
-
 Homo sapiens
-
3.6.1.13 peripheral blood
-
 Homo sapiens
-
3.6.1.13 pituitary gland high activity Homo sapiens
-
3.6.1.13 placenta high activity Homo sapiens
-
3.6.1.13 prostate
-
 Homo sapiens
-
3.6.1.13 salivary gland
-
 Homo sapiens
-
3.6.1.13 skeletal muscle
-
 Homo sapiens
-
3.6.1.13 small intestine
-
 Homo sapiens
-
3.6.1.13 spinal cord
-
 Homo sapiens
-
3.6.1.13 spleen
-
 Homo sapiens
-
3.6.1.13 stomach
-
 Homo sapiens
-
3.6.1.13 substantia nigra
-
 Homo sapiens
-
3.6.1.13 temporal lobe
-
 Homo sapiens
-
3.6.1.13 testis
-
 Homo sapiens
-
3.6.1.13 thalamus
-
 Homo sapiens
-
3.6.1.13 thymus high activity Homo sapiens
-
3.6.1.13 thyroid gland
-
 Homo sapiens
-
3.6.1.13 trachea
-
 Homo sapiens
-
3.6.1.13 uterus
-
 Homo sapiens
-
3.6.1.13 vermiform appendix
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.13 ADP-glucose + H2O 56% of the activity with ADP-ribose Homo sapiens AMP + alpha-D-glucose 1-phosphate
-
 ?
3.6.1.13 ADP-mannose + H2O 103% of the activity with ADP-ribose Homo sapiens AMP + D-mannose 1-phosphate
-
 ?
3.6.1.13 ADP-ribose + H2O
-
 Homo sapiens AMP + ribose 5-phosphate
-
 ?
3.6.1.13 ADP-ribose + H2O the function of the enzyme might be to remove free ADP-ribose arising from NAD+ and protein-bound poly- and non-enzymic protein glycation Homo sapiens AMP + ribose 5-phosphate
-
 ?
3.6.1.13 diadenosine 5',5''-diphosphate + H2O 20% the activity with ADP-ribose Homo sapiens ?
-
 ?
3.6.1.13 GDP-glucose + H2O 7% of the activity with ADP-ribose Homo sapiens GMP + glucose 5-phosphate
-
 ?
3.6.1.13 IDP-ribose + H2O 9% of the activity with ADP-ribose Homo sapiens IMP + ribose 5-phosphate
-
 ?
3.6.1.13 NAD+ + H2O 7% of the activity with ADP-ribose Homo sapiens AMP + NMN
-
 ?
3.6.1.13 NADH + H2O 7% of the activity with ADP-ribose Homo sapiens AMP + NMNH
-
 ?
3.6.1.13 NADPH + H2O 12% of the activity with ADP-ribose Homo sapiens AMP + ?
-
 ?
3.6.1.13 UDP-galactose + H2O 7% of the activity with ADP-ribose Homo sapiens UDP + galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.13 dimer 2 * 24300, native protein, mass spectrometry Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.6.1.13 adenosine 5'-diphosphosugar pyrophosphatase
-
 Homo sapiens
3.6.1.13 YSA1H
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.13 5.5
-
 ADP-ribose pH 7.5, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.13 7.4 9 with 2.5-5 mM Mg2+ or 0.1-0.25 mM Mn2+ Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6.1.13 0.02
-
 IC50: 0.02 mM Homo sapiens F-