EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.13 | 0.0005 | 0.001 | ADPribose | ADPRibase I | Rattus norvegicus | |
3.6.1.13 | 0.015 | 0.03 | ADPribose | ADPRibase-Mn | Rattus norvegicus | |
3.6.1.13 | 0.05 | 0.1 | ADPribose | ADPRibase II | Rattus norvegicus | |
3.6.1.53 | 0.015 | - |
ADP-ribose | pH 7.5, 37°C | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.13 | Mg2+ | ADPRibase-I is highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides when Mg2+ is replaced with Mn2+. ADPRibase-Mn is a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols. ADPribase-II is a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols | Rattus norvegicus | |
3.6.1.13 | Mn2+ | ADPRibase-I is highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides when Mg2+ is replaced with Mn2+. ADPRibase-Mn is a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols. ADPribase-II is a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols | Rattus norvegicus | |
3.6.1.53 | Mn2+ | required | Rattus norvegicus | |
3.6.1.53 | additional information | Mg2+ cannot substitute for Mn2+ | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.13 | 32000 | - |
ADPRibase-Mn, gel filtration | Rattus norvegicus |
3.6.1.13 | 40000 | - |
ADPRibase I, gel filtration | Rattus norvegicus |
3.6.1.13 | 47000 | - |
ADPRibase II, gel filtration | Rattus norvegicus |
3.6.1.53 | 32000 | - |
gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.13 | Rattus norvegicus | - |
- |
- |
3.6.1.53 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.13 | ADPRibaseI, ADPribase II and ADPRibase-Mn | Rattus norvegicus |
3.6.1.53 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.6.1.13 | liver | - |
Rattus norvegicus | - |
3.6.1.53 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.13 | 0.439 | - |
ADPRibase I | Rattus norvegicus |
3.6.1.13 | 12.81 | - |
ADPRibase II | Rattus norvegicus |
3.6.1.13 | 18.2 | - |
ADPRibase-Mn | Rattus norvegicus |
3.6.1.53 | 18.2 | - |
pH 7.5, 37°C | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.13 | ADP-glucose + H2O | ADPRibase II, very low activity with ADPRibase I | Rattus norvegicus | AMP + alpha-D-glucose 1-phosphate | - |
? | |
3.6.1.13 | ADP-mannose + H2O | ADPRibase II, low activity with ADPRibase I | Rattus norvegicus | AMP + D-mannose 1-phosphate | - |
? | |
3.6.1.13 | ADPribose + H2O | - |
Rattus norvegicus | AMP + D-ribose 5-phosphate | - |
? | |
3.6.1.13 | FAD + H2O | ADPRibase-Mn and ADPRibase II, very low activity with ADPRibase I in presence of Mn2+ | Rattus norvegicus | ? | - |
? | |
3.6.1.13 | IDP-ribose + H2O | - |
Rattus norvegicus | IMP + ribose 5-phosphate | - |
? | |
3.6.1.13 | additional information | three diphosphatases: 1.ADPRibase-I: an ADP-ribose diphosphatase highly specific for ADPribose and IDPribose in presence of Mg2+, but active also on non-reducing ADP-hexoses and dinucleotides, not on NAD+, when Mg2+ is replaced with Mn2+, 2. ADPRibase-Mn: a Mn2+-dependent diphosphatase active on ADPribose and IDPribose, dinucleotides and CDP alcohols, 3. ADPribase-II: a rather unspecific diphosphatase that, with Mg2+, is active on AMP or IMP containing NDP sugars and dinucleotides, not on NAD+, and with Mn2+ also active on non-adenine NDP-sugars and CDP-alcohols | Rattus norvegicus | ? | - |
? | |
3.6.1.13 | NAD+ + H2O | ADPRibase-Mn in presence of Mn2+, no activity with ADPRibase I and ADPRibase II | Rattus norvegicus | ? | - |
? | |
3.6.1.13 | NADH + H2O | ADPRibase I, ADPribase II and ADPRibase-Mn in presence of Mn2+ | Rattus norvegicus | ? | - |
? | |
3.6.1.53 | adenosine 5'-diphospho-5'-adenosine + H2O | - |
Rattus norvegicus | AMP | 45% of the activity with ADP-ribose | ? | |
3.6.1.53 | ADP + H2O | 27% of the activity with ADP-ribose | Rattus norvegicus | AMP + phosphate | - |
? | |
3.6.1.53 | ADP-ribose + H2O | - |
Rattus norvegicus | AMP + D-ribose 5-phosphate | - |
? | |
3.6.1.53 | CDP-choline + H2O | 140% of the activity with ADP-ribose | Rattus norvegicus | CMP + phosphocholine | - |
? | |
3.6.1.53 | CDP-ethanolamine + H2O | 89% of the activity with ADP-ribose | Rattus norvegicus | CMP + phosphoethanolamine | - |
? | |
3.6.1.53 | CDP-glycerol + H2O | 89% of the activity with ADP-ribose | Rattus norvegicus | CMP + phosphoglycerol | - |
? | |
3.6.1.53 | FAD + H2O | - |
Rattus norvegicus | ? | 39% of the activity with ADP-ribose | ? | |
3.6.1.53 | IDP-ribose + H2O | - |
Rattus norvegicus | IMP + D-ribose 5-phosphate | 105% of the activity with ADP-ribose | ? | |
3.6.1.53 | NAD+ + H2O | - |
Rattus norvegicus | ? | 23% of the activity with ADP-ribose | ? | |
3.6.1.53 | NADH + H+ + H2O | - |
Rattus norvegicus | ? | 25% of the activity with ADP-ribose | ? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.13 | 7 | 9 | ADPRibase I and ADPRibase II | Rattus norvegicus |
3.6.1.13 | 9 | - |
ADPRibase-Mn | Rattus norvegicus |
3.6.1.53 | 9 | - |
- |
Rattus norvegicus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.6.1.53 | Rattus norvegicus | isoelectric focusing | - |
4.3 |