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Literature summary extracted from

  • Allen, J.R.; Lasser, G.W.; Goldman, D.A.; Booth, J.W.; Mathews, C.K.
    T4 phage deoxyribonucleotide-synthesizing enzyme complex. Further studies on enzyme composition and regulation (1983), J. Biol. Chem., 258, 5746-5753.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.8 structural gene 42 encodes enzyme Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.8 27000
-
 2 * 27000 Tequatrovirus T4
2.1.2.8 60000
-
-
 Tequatrovirus T4
2.7.4.6 110000
-
-
 Escherichia coli
2.7.4.13 22000
-
-
 Tequatrovirus T4
3.6.1.12 59000
-
 dCTPase-dUTPase is an enzyme activity of the dNTP-synthesizing complex Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate Tequatrovirus T4 dCTP depresses the synthesis of 5-hydroxymethyldeoxycytidylate tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
 ?
2.1.2.8 additional information Tequatrovirus T4 enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus ?
-
 ?
2.7.4.11 ATP + dAMP Escherichia coli
-
 ADP + dADP
-
 ?
2.7.4.11 ATP + dAMP Escherichia coli 201
-
 ADP + dADP
-
 ?
2.7.4.13 ATP + deoxynucleoside phosphate Tequatrovirus T4 enzyme induced during viral infection ADP + deoxynucleoside diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.8 Tequatrovirus T4
-
 Escherichia coli infected with bacteriophage T4
-
2.7.4.6 Escherichia coli
-
 infected by bacteriophage T4, host-coded enzyme from infected Escherichia coli is part of bacteriophage T4 dNTP-synthesizing multi-enzyme complex
-
2.7.4.11 Escherichia coli
-
 infected with bacteriophage T4amBL292, a maturation defective phage mutant, host-coded activity which is a component of T4 dNTP-synthesizing enzyme complex
-
2.7.4.11 Escherichia coli 201
-
 infected with bacteriophage T4amBL292, a maturation defective phage mutant, host-coded activity which is a component of T4 dNTP-synthesizing enzyme complex
-
2.7.4.13 no activity in Escherichia coli
-
-
-
2.7.4.13 Tequatrovirus T4
-
 from infected Escherichia coli cells
-
2.7.4.13 Tequatrovirus T4
-
 maturation defective phage mutant T4amBL292, phage-coded activity which is a component of T4 dNTP-synthezising enzyme complex
-
3.6.1.12 Tequatrovirus T4
-
 dCTPase-dUTPase is an enzyme activity of the dNTP-synthesizing complex
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 partial Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
 Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
 ?
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate dCTP depresses the synthesis of 5-hydroxymethyldeoxycytidylate Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
 ?
2.1.2.8 additional information enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus Tequatrovirus T4 ?
-
 ?
2.7.4.6 NTP + nucleoside diphosphate
-
 Escherichia coli NDP + NTP
-
 ?
2.7.4.6 NTP + nucleoside diphosphate
-
 Escherichia coli 201 NDP + NTP
-
 ?
2.7.4.11 ATP + dAMP
-
 Escherichia coli ADP + dADP
-
 ?
2.7.4.11 ATP + dAMP
-
 Escherichia coli 201 ADP + dADP
-
 ?
2.7.4.13 ATP + dAMP
-
 Tequatrovirus T4 ADP + dADP
-
 ?
2.7.4.13 ATP + deoxynucleoside phosphate enzyme induced during viral infection Tequatrovirus T4 ADP + deoxynucleoside diphosphate
-
 ?
3.6.1.12 dCTP + H2O
-
 Tequatrovirus T4 dCMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.8 dimer 2 * 27000 Tequatrovirus T4
3.6.1.12 ? dCTPase-dUTPase is an enzyme activity of the dNTP-synthesizing complex, SDS-PAGE Tequatrovirus T4

Synonyms

EC Number Synonyms Comment Organism
3.6.1.12 dCTPase-dUTPase is an enzyme activity of the dNTP-synthesizing complex Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.8 5,10-methylenetetrahydrofolate
-
 Tequatrovirus T4
2.1.2.8 tetrahydrofolate
-
 Tequatrovirus T4