EC Number | General Stability | Organism |
---|---|---|
3.6.1.11 | repeated freezing and thawing destroys activity | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.11 | Ca2+ | competitive with respect to Mg2+ | Saccharomyces cerevisiae | |
3.6.1.11 | Cu2+ | - |
Saccharomyces cerevisiae | |
3.6.1.11 | diphosphate | competitive | Saccharomyces cerevisiae | |
3.6.1.11 | NaCl | 400 mM, about 41% loss of activity | Saccharomyces cerevisiae | |
3.6.1.11 | o-vanadate | 10 mM, nearly complete inhibition | Saccharomyces cerevisiae | |
3.6.1.11 | phosphate | no effect | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.11 | 0.0013 | - |
polyP33-36 | - |
Saccharomyces cerevisiae | |
3.6.1.11 | 0.033 | - |
polyP9-10 | - |
Saccharomyces cerevisiae | |
3.6.1.11 | 0.41 | - |
tripolyphosphate | - |
Saccharomyces cerevisiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.11 | Co2+ | 5 mM, 108% of the activation by Mg2+ | Saccharomyces cerevisiae | |
3.6.1.11 | Fe2+ | 5 mM, 34% of the activation by Mg2+ | Saccharomyces cerevisiae | |
3.6.1.11 | Mg2+ | required, optimal activity at 5 mM | Saccharomyces cerevisiae | |
3.6.1.11 | Ni2+ | 5 mM, 32% of the activation by Mg2+ | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.11 | 28000 | - |
gel filtration | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.11 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.11 | - |
Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.11 | additional information | - |
- |
Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
3.6.1.11 | -20°C, 50 mM Tris-HCl buffer, pH 7.6, 10 mM MgCl2, 0.5 mM EDTA, 150 mM NaCl, 20% loss of activity after 2 months | Saccharomyces cerevisiae |
3.6.1.11 | 4°C, in absence of detergents, complete loss of activity after 24 h, can be stabilized by 0.1% Triton X-100 and protease inhibitors | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.11 | (polyphosphate)n + H2O | poly P9-10, polyP33-36 | Saccharomyces cerevisiae | (polyphosphate)n-1 + phosphate | - |
? | |
3.6.1.11 | polyP33-36 | - |
Saccharomyces cerevisiae | polyP32-35 + phosphate | - |
? | |
3.6.1.11 | polyP9-10 | - |
Saccharomyces cerevisiae | polyP8-9 + phosphate | - |
? | |
3.6.1.11 | tripolyphosphate + H2O | - |
Saccharomyces cerevisiae | phosphate + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.11 | monomer | 1 * 28000, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.11 | 81 | - |
polyP33-36 | - |
Saccharomyces cerevisiae | |
3.6.1.11 | 90 | - |
polyP9-10 | - |
Saccharomyces cerevisiae | |
3.6.1.11 | 143 | - |
tripolyphosphate | - |
Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.11 | 7.5 | - |
- |
Saccharomyces cerevisiae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.11 | 6 | 9 | 45-55% of maximal activity at pH 6.0 and at pH 9.0 | Saccharomyces cerevisiae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.11 | 4 | - |
4°C, 48 h, 95% loss of activity | Saccharomyces cerevisiae |
3.6.1.11 | 5 | - |
4°C, 48 h, 66% loss of activity | Saccharomyces cerevisiae |
3.6.1.11 | 6 | - |
4°C, 48 h, 21% loss of activity | Saccharomyces cerevisiae |
3.6.1.11 | 7 | - |
4°C, 48 h, 5% loss of activity | Saccharomyces cerevisiae |
3.6.1.11 | 7.5 | 8 | 4°C, 48 h, stable | Saccharomyces cerevisiae |
3.6.1.11 | 9 | - |
4°C, 48 h, 11% loss of activity | Saccharomyces cerevisiae |
3.6.1.11 | 10 | - |
4°C, 48 h, 95% loss of activity | Saccharomyces cerevisiae |