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Literature summary extracted from

  • Afansieva, T.P.; Kulaev, I.S.
    Polyphosphate phosphohydrolase from Endomyces magnusii (1973), Biochim. Biophys. Acta, 321, 336-347.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.6.1.11 loss of activity after dialysis Magnusiomyces magnusii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.11 Ag+
-
 Magnusiomyces magnusii
3.6.1.11 Al3+
-
 Magnusiomyces magnusii
3.6.1.11 Ca2+
-
 Magnusiomyces magnusii
3.6.1.11 Cd2+
-
 Magnusiomyces magnusii
3.6.1.11 Cu2+
-
 Magnusiomyces magnusii
3.6.1.11 EDTA
-
 Magnusiomyces magnusii
3.6.1.11 Hg2+
-
 Magnusiomyces magnusii
3.6.1.11 KCN
-
 Magnusiomyces magnusii
3.6.1.11 NaF
-
 Magnusiomyces magnusii
3.6.1.11 Pb2+
-
 Magnusiomyces magnusii
3.6.1.11 PCMB
-
 Magnusiomyces magnusii
3.6.1.11 phosphate
-
 Magnusiomyces magnusii
3.6.1.11 UO22+
-
 Magnusiomyces magnusii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.11 additional information
-
 additional information
-
 Magnusiomyces magnusii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.11 Co2+ Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective Magnusiomyces magnusii
3.6.1.11 Fe2+ Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective Magnusiomyces magnusii
3.6.1.11 Mg2+ Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective Magnusiomyces magnusii
3.6.1.11 Mn2+ Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective Magnusiomyces magnusii
3.6.1.11 Ni2+ Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective Magnusiomyces magnusii
3.6.1.11 Zn2+ Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective Magnusiomyces magnusii

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.11 Magnusiomyces magnusii
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.6.1.11 -20°C, stable for months Magnusiomyces magnusii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.11 (polyphosphate)n + H2O n = 40, 72, 180 or 290, highest reaction rate, when n is 40 Magnusiomyces magnusii (polyphosphate)n-1 + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.11 37
-
-
 Magnusiomyces magnusii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.11 30 48 30°C: about about 60% of maximal activity, 48°C: about 40% of maximal activity Magnusiomyces magnusii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.11 40
-
 15 min, 70% loss of activity Magnusiomyces magnusii
3.6.1.11 60
-
 2 min, complete loss of activity, slight protection from heat inactivation, 10%, by 1 mM MnCl2 Magnusiomyces magnusii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.11 7.1
-
-
 Magnusiomyces magnusii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.11 6.2 8.4 pH 6.2: about 35% of maximal activity, pH 8.4: about 60% of maximal activity Magnusiomyces magnusii