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Literature summary extracted from

  • Kumble, K.; Kornberg, A.
    Endopolyphosphatase for long chain inorganic polyphosphate in yeast and mammals (1996), J. Biol. Chem., 271, 27146-27151.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.10 CaCl2 1 mM, 35% inhibition Saccharomyces cerevisiae
3.6.1.10 diphosphate 10 mM, complete inhibition Saccharomyces cerevisiae
3.6.1.10 EDTA 10 mM, complete inhibition Saccharomyces cerevisiae
3.6.1.10 phosphate 20 mM, 50% inhibition Saccharomyces cerevisiae
3.6.1.10 ZnCl2 1 mM, 35% inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.10 0.000185
-
 polyP750
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.10 vacuole
-
 Saccharomyces cerevisiae 5773
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.10 Mg2+
-
 Neurospora crassa
3.6.1.10 Mg2+ activates Saccharomyces cerevisiae
3.6.1.10 Mn2+
-
 Neurospora crassa
3.6.1.10 Mn2+ optimal activation at 2.5 mM Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.10 35000
-
 2 * 35000, SDS-PAGE Saccharomyces cerevisiae
3.6.1.10 80000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.10 Caenorhabditis elegans
-
-
-
3.6.1.10 Chlamydomonas sp.
-
 low activity
-
3.6.1.10 Dictyostelium discoideum
-
-
-
3.6.1.10 Drosophila melanogaster
-
-
-
3.6.1.10 Giardia intestinalis
-
 low activity
-
3.6.1.10 Neurospora crassa
-
-
-
3.6.1.10 Phaeodactylum tricornutum
-
 low activity
-
3.6.1.10 Pyrococcus islandicum
-
 low activity
-
3.6.1.10 Rattus norvegicus
-
-
-
3.6.1.10 Saccharomyces cerevisiae
-
-
-
3.6.1.10 Sulfolobus acidocaldarius
-
 low activity
-
3.6.1.10 Synechococcus sp.
-
 low activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.10
-
 Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.10 brain
-
 Rattus norvegicus
-
3.6.1.10 heart low activity Rattus norvegicus
-
3.6.1.10 kidney low activity Rattus norvegicus
-
3.6.1.10 liver low activity Rattus norvegicus
-
3.6.1.10 lung low activity Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.10 polyP750 + H2O
-
 Saccharomyces cerevisiae polyP749 + phosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Drosophila melanogaster oligophosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Rattus norvegicus oligophosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Neurospora crassa oligophosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Giardia intestinalis oligophosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Dictyostelium discoideum oligophosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Sulfolobus acidocaldarius oligophosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Caenorhabditis elegans oligophosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Chlamydomonas sp. oligophosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Synechococcus sp. oligophosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Phaeodactylum tricornutum oligophosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Pyrococcus islandicum oligophosphate
-
 ?
3.6.1.10 polyphosphate + H2O
-
 Saccharomyces cerevisiae oligophosphate distributive action on polyP750 produces shorter chains to a limit of about polyP60 as well as the more abundant release of polyP3, limit digestion product is polyP3 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.10 dimer 2 * 35000, SDS-PAGE Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.10 20
-
 phosphate
-
 Saccharomyces cerevisiae