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Literature summary extracted from

  • Kuhn, N.J.; Wadeson, A.; Ward, S.; Young, T.W.
    Methanococcus jannaschii ORF mj0608 codes for a class C inorganic pyrophosphatase protected by Co2+ or Mn2+ ions against fluoride inhibition (2000), Arch. Biochem. Biophys., 379, 292-298.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 expressed in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 Cd2+
-
 Methanocaldococcus jannaschii
3.6.1.1 Cu2+
-
 Methanocaldococcus jannaschii
3.6.1.1 F- Co2+ and Mn2+ protect against fluoride inhibition Methanocaldococcus jannaschii
3.6.1.1 Fe2+
-
 Methanocaldococcus jannaschii
3.6.1.1 Zn2+ inhibition caused at a separate site rather than as Zn-diphosphate substrate Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Co2+ protects against fluoride inhibition Methanocaldococcus jannaschii
3.6.1.1 Mg2+
-
 Methanocaldococcus jannaschii
3.6.1.1 Mn2+ protects against fluoride inhibition Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Methanocaldococcus jannaschii
-
 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1
-
 Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.1 600
-
 at 25°C Methanocaldococcus jannaschii
3.6.1.1 8000
-
 at 85°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
 Methanocaldococcus jannaschii 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O high specificity Methanocaldococcus jannaschii 2 phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 85
-
 growth optimum for organism and highest activity Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 9
-
 assay at Methanocaldococcus jannaschii