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Literature summary extracted from

  • Bandypadhyay, A.K.; Nagasawa, T.; Asano, Y.; Fujishiro, K.; Tani, Y.; Yamada, H.
    Purification and characterization of benzonitrilases from Arthrobacter sp. strain J-1 (1986), Appl. Environ. Microbiol., 51, 302-306.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 4-chloromercuribenzoate 91-100% inhibition at 0.1 mM Arthrobacter sp.
3.5.5.1 Ag+ complete inhibition at 0.1 mM Arthrobacter sp.
3.5.5.1 CaCl2 inhibition at 50 mM Arthrobacter sp.
3.5.5.1 Cu2+ benzonitrilase A: 30% inhibition at 0.1 mM, benzonitrilase B: 83% inhibition at 0.1 mM Arthrobacter sp.
3.5.5.1 Hg2+ complete inhibition at 0.1 mM Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 4.5
-
 Benzonitrile benzonitrilase B Arthrobacter sp.
3.5.5.1 6.7
-
 Benzonitrile benzonitrilase A Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 23000
-
 gel filtration, benzonitrilase B Arthrobacter sp.
3.5.5.1 30000
-
 gel filtration, benzonitrilase A Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Arthrobacter sp.
-
-
-
3.5.5.1 Arthrobacter sp. J-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 benzonitrilase A, to homogeneity, partial for benzonitrilase B Arthrobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.1 cell culture use of benzonitrile as a sole source of nitrogen and carbon Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.1 0.12
-
 benzonitrilase B Arthrobacter sp.
3.5.5.1 1.31
-
 benzonitrilase A Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 4-chlorobenzonitrile + H2O
-
 Arthrobacter sp. 4-chlorobenzoic acid + NH3
-
 ?
3.5.5.1 4-chlorobenzonitrile + H2O
-
 Arthrobacter sp. J-1 4-chlorobenzoic acid + NH3
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O best substrate Arthrobacter sp. 4-pyridinecarboxylic acid + NH3
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O best substrate Arthrobacter sp. J-1 4-pyridinecarboxylic acid + NH3
-
 ?
3.5.5.1 4-toluonitrile + H2O
-
 Arthrobacter sp. NH3 + 4-methylbenzoate
-
 ?
3.5.5.1 4-toluonitrile + H2O
-
 Arthrobacter sp. J-1 NH3 + 4-methylbenzoate
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Arthrobacter sp. benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Arthrobacter sp. J-1 benzoic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 monomer 1 * 30000, benzonitrilase A, SDS-PAGE Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 benzonitrilase A
-
 Arthrobacter sp.
3.5.5.1 benzonitrilase B
-
 Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 30
-
 benzonitrilase B Arthrobacter sp.
3.5.5.1 40
-
 benzonitrilase A Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.1 30
-
 benzonitrilase B: gradually degraded above Arthrobacter sp.
3.5.5.1 45
-
 benzonitrilase A, stable up to Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 7.5
-
 benzonitrilase B Arthrobacter sp.
3.5.5.1 8.5
-
 benzonitrilase A Arthrobacter sp.