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Literature summary extracted from

  • Kobayashi, M.; Goda, M.; Shimizu, S.
    Nitrilase catalyzes amide hydrolysis as well as nitrile hydrolysis (1998), Biochem. Biophys. Res. Commun., 253, 662-666.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expression in Escherichia coli Rhodococcus rhodochrous

Protein Variants

EC Number Protein Variants Comment Organism
3.5.5.1 C165A no hydrolysis of benzamide Rhodococcus rhodochrous
3.5.5.1 C165S no hydrolysis of benzamide Rhodococcus rhodochrous

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 Benzamide competitive inhibition of benzonitrile hydrolysis, Ki: 16.6 mM Rhodococcus rhodochrous

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 2.1
-
 Benzonitrile
-
 Rhodococcus rhodochrous
3.5.5.1 2.94
-
 Benzamide
-
 Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 benzonitrile + 2 H2O Rhodococcus rhodochrous
-
 benzoic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Rhodococcus rhodochrous
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 highly purified, recombinant enzyme Rhodococcus rhodochrous

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.1 cell culture
-
 Rhodococcus rhodochrous
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.1 15.3
-
 for benzonitrile as substrate, for benzamide as substrate very low, but significant specific acitivity Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 benzamide + H2O catalysed by a highly purified nitrilase, but very low activity Rhodococcus rhodochrous benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Rhodococcus rhodochrous benzoic acid + NH3
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.5.1 16.6
-
 Benzamide competitive inhibition of benzonitrile hydrolysis Rhodococcus rhodochrous