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Literature summary extracted from

  • Schmidt, A.; Wu, H.; MacKenzie, R.E.; Chen, V.J.; Bewly, J.R.; Ray, J.E.; Toth, J.E; Cygler, M.
    Structures of three inhibitor complexes provide insight into the reaction mechanism of the human methylenetetrahydrofolate dehydrogenase/cyclohydrolase (2000), Biochemistry, 39, 6325-6335.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.4.9 medicine enzyme represents a potential pharmaceutical target for manipulation of cell growth and development and hence cancer prevention or treatment Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.9 crystal structure of DC301, the cytosolic DC domain of the trifunctional enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.9 0.14
-
 5,10-methenyltetrahydrofolate mutant K56R Homo sapiens
3.5.4.9 0.32
-
 5,10-methenyltetrahydrofolate mutant S49Q Homo sapiens
3.5.4.9 0.34
-
 5,10-methenyltetrahydrofolate wild-type DC301 Homo sapiens
3.5.4.9 0.48
-
 5,10-methenyltetrahydrofolate mutant Y52F Homo sapiens
3.5.4.9 0.52
-
 5,10-methenyltetrahydrofolate mutant S49A Homo sapiens
3.5.4.9 0.56
-
 5,10-methenyltetrahydrofolate mutants C147A, T148A Homo sapiens
3.5.4.9 1.22
-
 5,10-methenyltetrahydrofolate mutant Y52A Homo sapiens
3.5.4.9 1.62
-
 5,10-methenyltetrahydrofolate mutant Y52F Homo sapiens
3.5.4.9 2.36
-
 5,10-methenyltetrahydrofolate mutant C147Q Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Drosophila melanogaster
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Mus musculus
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Rattus norvegicus
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Homo sapiens
-
 10-formyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.9 Drosophila melanogaster
-
-
-
3.5.4.9 Homo sapiens P11586
-
-
3.5.4.9 Mus musculus
-
-
-
3.5.4.9 Rattus norvegicus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.9 1.6
-
 mutant K56R Homo sapiens
3.5.4.9 6.7
-
 mutants C147Q, S49Q Homo sapiens
3.5.4.9 18
-
 mutant C147Q Homo sapiens
3.5.4.9 25
-
 mutant S49Q Homo sapiens
3.5.4.9 39
-
 mutant Y52S Homo sapiens
3.5.4.9 47
-
 mutant Y52A Homo sapiens
3.5.4.9 128
-
 mutantS49A Homo sapiens
3.5.4.9 133
-
 DC301, wild-type Homo sapiens
3.5.4.9 146
-
 mutants C147A, T148A Homo sapiens
3.5.4.9 160
-
 mutant Y52F Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Drosophila melanogaster 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Mus musculus 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Rattus norvegicus 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Homo sapiens 10-formyltetrahydrofolate
-
 ?