Any feedback?
Literature summary extracted from
- Methylenetetrahydrofolate dehydrogenase-cyclohydrolase from Photobacterium phosphoreum shares properties with a mammalian mitochondrial homologue (1996), Biochim. Biophys. Acta, 1296, 47-54.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | phosphate | effect on the NAD+-dependent dehydrogenase activity | Photobacterium phosphoreum |  |
| 3.5.4.9 | phosphate | - |
Homo sapiens | |
| 3.5.4.9 | phosphate | - |
Photobacterium phosphoreum | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.1.5 | expressed in Escherichia coli | Photobacterium phosphoreum |
| 3.5.4.9 | amplified genomic library transformed into yest YA3-1, yeast-DNA transformed into Escherichia coli K38 pBKe, gene folD encoding PPDC expressed | Photobacterium phosphoreum |
| 3.5.4.9 | bifunctional dehydrogenase/cyclohydrolase portion of the cytoplasmic trifunctional enzyme cloned and heterologous expressed in the yeast strain YA3-1 | Homo sapiens |
| 3.5.4.9 | cDNA encoding trifunctional enzyme used to complete the purine auxotrophic yeast strain YA3-1 | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | potassium phosphate | effect on NADP+-dependent dehydrogenase activity | Photobacterium phosphoreum | |
| 1.5.1.5 | potassium sulfate | effect on NADP+-dependent dehydrogenase activity | Photobacterium phosphoreum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.5 | 0.0011 | - |
5,10-methylenetetrahydrofolate | cofactor: NADP+ | Photobacterium phosphoreum | |
| 1.5.1.5 | 0.016 | - |
5,10-methylenetetrahydrofolate | cofactor: NAD+ | Photobacterium phosphoreum | |
| 1.5.1.5 | 0.2 | - |
NADP+ | - |
Photobacterium phosphoreum | |
| 1.5.1.5 | 4.76 | - |
5,10-methylenetetrahydrofolate | - |
Photobacterium phosphoreum | |
| 3.5.4.9 | 0.031 | - |
(6R,S)-methenyltetrahydrofolate | - |
Photobacterium phosphoreum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 31000 | - |
x * 31000, SDS-PAGE | Photobacterium phosphoreum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | Photobacterium phosphoreum | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Drosophila melanogaster | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Mus musculus | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Escherichia coli | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Homo sapiens | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Rattus norvegicus | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Saccharomyces cerevisiae | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Photobacterium phosphoreum | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Clostridium formicaceticum | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Spodoptera frugiperda | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Acetobacterium woodii | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Spodoptera frugiperda Sf9 | - |
10-formyltetrahydrofolate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.5 | Photobacterium phosphoreum | - |
- |
- |
| 3.5.4.9 | Acetobacterium woodii | - |
bifunctional enzyme | - |
| 3.5.4.9 | Clostridium formicaceticum | - |
ATCC 23439, monofunctional enzyme | - |
| 3.5.4.9 | Drosophila melanogaster | - |
- |
- |
| 3.5.4.9 | Escherichia coli | - |
- |
- |
| 3.5.4.9 | Homo sapiens | - |
human, bifunctional mitochondrial enzyme, trifunctional cytoplasmic enzyme | - |
| 3.5.4.9 | Mus musculus | - |
mouse, bifunctional mitochondrial enzyme | - |
| 3.5.4.9 | Photobacterium phosphoreum | - |
marine bioluminescent bacterium, bifunctional enzyme | - |
| 3.5.4.9 | Rattus norvegicus | - |
rat, trifunctional cytoplasmic enzyme | - |
| 3.5.4.9 | Saccharomyces cerevisiae | - |
cytoplasmic, mitochondrial | - |
| 3.5.4.9 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| 3.5.4.9 | Spodoptera frugiperda | - |
- |
- |
| 3.5.4.9 | Spodoptera frugiperda Sf9 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.5 | bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase | Photobacterium phosphoreum |
| 3.5.4.9 | recombinant protein expressed in Escherichia coli K38 | Photobacterium phosphoreum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 97.4 | - |
recombinant bifunctional enzyme | Photobacterium phosphoreum |
| 3.5.4.9 | 97.4 | - |
recombinant enzyme | Photobacterium phosphoreum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.5 | 5,10-methylenetetrahydrofolate + NAD+ | - |
Photobacterium phosphoreum | 5,10-methenyltetrahydrofolate + NADH + H+ | - |
r | |
| 1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | - |
Photobacterium phosphoreum | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
| 3.5.4.9 | (6R,S)-methenyltetrahydrofolate + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.5.4.9 | (6R,S)-methenyltetrahydrofolate + H2O | - |
Photobacterium phosphoreum | ? | - |
r | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Drosophila melanogaster | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Mus musculus | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Homo sapiens | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Rattus norvegicus | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Saccharomyces cerevisiae | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Photobacterium phosphoreum | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Clostridium formicaceticum | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Spodoptera frugiperda | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Acetobacterium woodii | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Spodoptera frugiperda Sf9 | 10-formyltetrahydrofolate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.1.5 | ? | x * 31000, SDS-PAGE | Photobacterium phosphoreum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.5 | 113 | - |
NADP+ | - |
Photobacterium phosphoreum | |
| 1.5.1.5 | 136 | - |
NAD+ | - |
Photobacterium phosphoreum | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | NAD+ | NADP+ preferred cofactor in vivo | Photobacterium phosphoreum | |
| 1.5.1.5 | NADP+ | - |
Photobacterium phosphoreum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
