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Literature summary extracted from

  • Rabinowitz, J.C.; Pricer, W.E.
    Purine fermentation by Clostridium cylindrosporum. V. Formiminoglycine (1956), J. Biol. Chem., 222, 537-554.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.4.8 unstable in absence of reducing agents, completely inactive without cysteine, cysteine, sodium hydrosulfite, glutathione, sodium thioglycolate and ascorbic acid stabilizes enzyme activity Clostridium cylindrosporum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.8 3-Amino-1,2,4-triazole
-
 Clostridium cylindrosporum
3.5.4.8 4-Hydroxy-1,2,3-triazole less effective inhibitor Clostridium cylindrosporum
3.5.4.8 Co2+ addition of 0.002 M reduces activity by 50% or more Clostridium cylindrosporum
3.5.4.8 EDTA
-
 Clostridium cylindrosporum
3.5.4.8 histamine less effective inhibitor Clostridium cylindrosporum
3.5.4.8 Mg2+ addition of 0.002 M reduces activity by 50% or more Clostridium cylindrosporum
3.5.4.8 Mn2+ addition of 0.002 M reduces activity by 50% or more Clostridium cylindrosporum
3.5.4.8 Zn2+ addition of 0.002 M reduces activity by 50% or more Clostridium cylindrosporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.8 1.8
-
 4-aminoimidazole
-
 Clostridium cylindrosporum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.8 Co2+ restores activity of EDTA treated enzyme to 83% of the original activity Clostridium cylindrosporum
3.5.4.8 Fe2+ divalent cation required for activity Clostridium cylindrosporum
3.5.4.8 Mn2+ restores activity of EDTA treated enzyme to 60% of the original activity Clostridium cylindrosporum
3.5.4.8 Ni2+ restores activity of EDTA treated enzyme to 23% of the original activity Clostridium cylindrosporum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.8 4-aminoimidazole + H2O Clostridium cylindrosporum
-
 formiminoglycine + NH3 + unidentified product alkali-labile derivative of glycine, can be hydrolyzed to yield glycine ?
3.5.4.8 4-aminoimidazole + H2O Clostridium cylindrosporum HC1
-
 formiminoglycine + NH3 + unidentified product alkali-labile derivative of glycine, can be hydrolyzed to yield glycine ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.8 Clostridium cylindrosporum
-
-
-
3.5.4.8 Clostridium cylindrosporum HC1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.8
-
 Clostridium cylindrosporum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.8 140
-
-
 Clostridium cylindrosporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.8 4-aminoimidazole + H2O
-
 Clostridium cylindrosporum formiminoglycine + NH3 + unidentified product
-
 ?
3.5.4.8 4-aminoimidazole + H2O
-
 Clostridium cylindrosporum formiminoglycine + NH3 + unidentified product alkali-labile derivative of glycine, can be hydrolyzed to yield glycine ?
3.5.4.8 4-aminoimidazole + H2O
-
 Clostridium cylindrosporum HC1 formiminoglycine + NH3 + unidentified product
-
 ?
3.5.4.8 4-aminoimidazole + H2O
-
 Clostridium cylindrosporum HC1 formiminoglycine + NH3 + unidentified product alkali-labile derivative of glycine, can be hydrolyzed to yield glycine ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.8 7
-
 in triethanolamine hydrochloride Clostridium cylindrosporum