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Literature summary extracted from

  • Kwon, T.K.; Lee, S.Y.; Kim, J.G.; Song, B.H.; Hong, S.D.
    Purification and properties of Escherichia coli cytidine deaminase by amplification of the cdd gene (1991), Mol. Cells, 1, 281-286.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.5 cdd gene cloned and amplified with pTK148, cloning vector pBR322, enzyme overexpression in Escherichia coli C600 Escherichia coli
3.5.4.5 cdd genes cloned and characterized Salmonella enterica subsp. enterica serovar Typhimurium
3.5.4.5 cdd genes cloned and characterized Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.5 3,4,5,6-Tetrahydrouridine
-
 Escherichia coli
3.5.4.5 5,6-Dihydrouridine competitive inhibition Escherichia coli
3.5.4.5 5-bromodeoxyuridine
-
 Escherichia coli
3.5.4.5 adenosine
-
 Escherichia coli
3.5.4.5 Cu2+
-
 Escherichia coli
3.5.4.5 deoxyadenosine competitive inhibition Escherichia coli
3.5.4.5 deoxyguanosine
-
 Escherichia coli
3.5.4.5 deoxyuridine competitive inhibition Escherichia coli
3.5.4.5 Fe2+
-
 Escherichia coli
3.5.4.5 Fe3+
-
 Escherichia coli
3.5.4.5 guanosine
-
 Escherichia coli
3.5.4.5 Hg+
-
 Escherichia coli
3.5.4.5 p-chloromercuribenzoate
-
 Saccharomyces cerevisiae
3.5.4.5 thioglycolic acid 0.7 mM 88% relative activity, complete inhibition at 7 mM Escherichia coli
3.5.4.5 thymidine competitive inhibition Escherichia coli
3.5.4.5 uridine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 0.0167
-
 cytidine nonhomogenous enzyme Escherichia coli
3.5.4.5 0.089
-
 cytosine deoxyriboside
-
 Escherichia coli
3.5.4.5 0.21
-
 deoxycytidine amplified gene Escherichia coli
3.5.4.5 0.58
-
 deoxycytidine
-
 Escherichia coli
3.5.4.5 2.2
-
 cytidine homogenous preparation Escherichia coli
3.5.4.5 6.6
-
 cytidine amplified gene, pH 7.0, 37°C Escherichia coli
3.5.4.5 8.53
-
 deoxycytidine
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.5 cytoplasm inner-surface of cytoplasmic membrane Escherichia coli 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 14000
-
 4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments Bacillus subtilis
3.5.4.5 33000
-
 2 * 33000, SDS-PAGE Escherichia coli
3.5.4.5 34000
-
 2 * 34000, SDS-PAGE Escherichia coli
3.5.4.5 54000
-
-
 Escherichia coli
3.5.4.5 56000
-
 calculated from Stokes radius Bacillus subtilis
3.5.4.5 56000
-
 calculated from Stokes radius Escherichia coli
3.5.4.5 57000
-
 gel filtration, homogenous preparation Escherichia coli
3.5.4.5 62000
-
 gel filtration, native enzyme Escherichia coli
3.5.4.5 73000
-
 gel filtration, nonhomogenous preparation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.5 cytidine + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Bacillus subtilis
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Escherichia coli
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Saccharomyces cerevisiae
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Geobacillus stearothermophilus
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Escherichia coli JF611
-
 uridine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Bacillus subtilis
-
-
-
3.5.4.5 Escherichia coli
-
-
-
3.5.4.5 Escherichia coli JF611
-
-
-
3.5.4.5 Geobacillus stearothermophilus
-
-
-
3.5.4.5 Saccharomyces cerevisiae
-
 bakers' yeast
-
3.5.4.5 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.5 4
-
 amplified gene, 5'-aza-2'-deoxycytidine as substrate Escherichia coli
3.5.4.5 4.9
-
 amplified gene, 2'-thiocytidine as substrate Escherichia coli
3.5.4.5 23.9
-
 amplified gene, 5'-methylcytidine as substrate Escherichia coli
3.5.4.5 31.8
-
 amplified gene, ara-cytidine as substrate Escherichia coli
3.5.4.5 43
-
 amplified gene, cytidine as substrate Escherichia coli
3.5.4.5 47.9
-
 amplified gene, 5'-methyl-2'-deoxycytidine as substrate Escherichia coli
3.5.4.5 68.4
-
 amplified gene, 2'-fluoro-2'-deoxycytidine as substrate Escherichia coli
3.5.4.5 97.6
-
 amplified gene, deoxycytidine as substrate Escherichia coli
3.5.4.5 3558
-
 amplified gene Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 1-beta-D-arabinofuranosylcytosine + H2O
-
 Escherichia coli ?
-
 ?
3.5.4.5 2'-deoxycytidine + H2O
-
 Escherichia coli 2'-deoxyuridine + NH3
-
 ?
3.5.4.5 2'-deoxycytidine + H2O
-
 Saccharomyces cerevisiae 2'-deoxyuridine + NH3
-
 ?
3.5.4.5 2'-deoxycytidine + H2O
-
 Escherichia coli JF611 2'-deoxyuridine + NH3
-
 ?
3.5.4.5 2'-fluoro-2'-deoxycytidine + H2O
-
 Escherichia coli 2'-fluoro-2'-deoxyuridine + NH3
-
 ?
3.5.4.5 2'-fluoro-2'-deoxycytidine + H2O
-
 Escherichia coli JF611 2'-fluoro-2'-deoxyuridine + NH3
-
 ?
3.5.4.5 2'-thio-cytidine + H2O
-
 Escherichia coli 2'-thiouridine + NH3
-
 ?
3.5.4.5 5'-methyl-2'-deoxycytidine + H2O
-
 Escherichia coli 5'-methyl-2-deoxyuridine + NH3
-
 ?
3.5.4.5 5'-methyl-2'-deoxycytidine + H2O
-
 Escherichia coli JF611 5'-methyl-2-deoxyuridine + NH3
-
 ?
3.5.4.5 5-aza-2'-deoxycytidine + H2O
-
 Escherichia coli 5-aza-2'-deoxyuridine + NH3
-
 ?
3.5.4.5 5-methylcytidine + H2O
-
 Escherichia coli 5-methyluridine + NH3
-
 ?
3.5.4.5 5-methylcytidine + H2O
-
 Escherichia coli JF611 5-methyluridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Bacillus subtilis uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Saccharomyces cerevisiae uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Geobacillus stearothermophilus uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli JF611 uridine + NH3
-
 ?
3.5.4.5 cytosine deoxyriboside + H2O
-
 Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.5 dimer 2 * 34000, SDS-PAGE Escherichia coli
3.5.4.5 dimer 2 * 33000, SDS-PAGE Escherichia coli
3.5.4.5 tetramer 4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.5 30
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.5.4.5 30
-
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.5 60
-
 relatively labile to thermal treatment, complete inactivation by incubation for 30 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.5 7
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.5.4.5 7
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.4.5 6 9 reasonably stable in this range Escherichia coli