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Literature summary extracted from

  • Vita, A.; Amici, A.; Cacciamani, T.; Lanciotti, M.; Magni, G.
    Cytidine deaminase from Escherichia coli B. Purification and enzymatic and molecular properties (1985), Biochemistry, 24, 6020-6024.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.5 1-methyladenosine competitive inhibition Escherichia coli
3.5.4.5 5,6-Dihydrouridine
-
 Escherichia coli
3.5.4.5 deoxyadenosine competitive inhibition Escherichia coli
3.5.4.5 deoxyuridine competitive inhibition Escherichia coli
3.5.4.5 dihydrothymidine competitive inhibition Escherichia coli
3.5.4.5 NH3 product inhibition Escherichia coli
3.5.4.5 thymidine competitive inhibition Escherichia coli
3.5.4.5 thymine riboside competitive inhibition Escherichia coli
3.5.4.5 uridine product inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 0.09
-
 deoxycytidine
-
 Escherichia coli
3.5.4.5 0.18
-
 cytidine
-
 Escherichia coli
3.5.4.5 1.25
-
 5-methylcytidine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 33000
-
 2 * 33000, SDS-PAGE Escherichia coli
3.5.4.5 56000
-
 gel filtration, Sephacryl S-300 Escherichia coli
3.5.4.5 66000
-
 gel filtration, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.5 cytidine + H2O Escherichia coli
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Escherichia coli B / ATCC 11303
-
 uridine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Escherichia coli
-
-
-
3.5.4.5 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.5.4.5 -20°C, 50 mM phosphate buffer pH7.5 after 1 month pure enzyme retains 70% original activity Escherichia coli
3.5.4.5 -20°C, 50 mM Tris-HCl, pH7.5, after 1 month pure enzyme retains 80% original activity Escherichia coli
3.5.4.5 4°C, 50 mM Tris-HCl, pH 7.5, pure enzyme retains 63% original activity after 2 weeks Escherichia coli
3.5.4.5 4°C,50 mM phosphate buffer pH 7.5, pure enzyme retains 13% original activity after 2 weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 5-methylcytidine + H2O
-
 Escherichia coli 5-methyluridine + NH3
-
 ?
3.5.4.5 5-methylcytidine + H2O
-
 Escherichia coli B / ATCC 11303 5-methyluridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli B / ATCC 11303 uridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Escherichia coli deoxyuridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Escherichia coli B / ATCC 11303 deoxyuridine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.5 dimer 2 * 33000, SDS-PAGE Escherichia coli