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Literature summary extracted from

  • Pugh, M.E.; Bieber, A.L.
    Studies of rabbit brain, intestine and liver guanine aminohydrolase (1984), Comp. Biochem. Physiol. B, 77, 619-627.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.4.3 medicine enzyme has been implicated in cancer, liver disease, saturine gout, psoriasis and autoimmune disease, histochemical method for demonstration of guanase should be useful clinically, guanase activity increases significantly in lung and gastric cancer tissues or in patients with liver diseases like hepatitis, guanase is a biochemical indicator of rejection in liver transplant recipients, serum enzyme activity is the most sensitive indicator of liver disease, inhibition of guanase has beneficial implications in cancer chemotherapy, an inhibitor as chemotherapeutic agent selectively inhibit the growth of rapidly proliferating cancerous cells via depletion of the purine nucleotide pool Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.3 5-aminoimidazole-4-carboxamide
-
 Homo sapiens
3.5.4.3 5-aminoimidazole-4-carboxamide
-
 Oryctolagus cuniculus
3.5.4.3 F-
-
 Homo sapiens
3.5.4.3 F-
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.3 0.00296
-
 guanine brain enzyme at pH 5.5 Oryctolagus cuniculus
3.5.4.3 0.0033
-
 guanine liver enzyme at pH 9.0 Oryctolagus cuniculus
3.5.4.3 0.0035
-
 guanine brain enzyme at pH 9.0 Oryctolagus cuniculus
3.5.4.3 0.0036
-
 guanine intestine enzyme at pH 9.0 Oryctolagus cuniculus
3.5.4.3 0.0038
-
 guanine at pH 7 , intestine enzyme at pH 5.5 Oryctolagus cuniculus
3.5.4.3 0.00451
-
 guanine intestine enzyme at pH 5.5 Oryctolagus cuniculus
3.5.4.3 0.00476
-
 guanine liver enzyme at pH 5.1 Oryctolagus cuniculus
3.5.4.3 0.00478
-
 guanine intestine enzyme at pH 5.1 Oryctolagus cuniculus
3.5.4.3 0.00481
-
 guanine brain enzyme at pH 5.1, liver enzyme at pH 7.0 Oryctolagus cuniculus
3.5.4.3 0.00509
-
 guanine intestine enzyme at pH 7.0 Oryctolagus cuniculus
3.5.4.3 0.00518
-
 guanine liver enzyme at pH 8.5 Oryctolagus cuniculus
3.5.4.3 0.00527
-
 guanine liver enzyme at pH 6.0 Oryctolagus cuniculus
3.5.4.3 0.00546
-
 guanine brain enzyme at pH 8.5 Oryctolagus cuniculus
3.5.4.3 0.0056
-
 guanine
-
 Homo sapiens
3.5.4.3 0.0056
-
 guanine at pH 8 Oryctolagus cuniculus
3.5.4.3 0.0058
-
 guanine liver enzyme at pH 8.0 Oryctolagus cuniculus
3.5.4.3 0.00586
-
 guanine brain enzyme at pH 6.0 Oryctolagus cuniculus
3.5.4.3 0.0061
-
 guanine brain and intestine enzymes at pH 8 Oryctolagus cuniculus
3.5.4.3 0.00611
-
 guanine brain and intestine enzymes at pH 8 Oryctolagus cuniculus
3.5.4.3 0.0063
-
 guanine brain enzyme at pH 7.0 Oryctolagus cuniculus
3.5.4.3 0.00671
-
 guanine intestine enzyme at pH 8.5 Oryctolagus cuniculus
3.5.4.3 0.0068
-
 guanine at pH 7.4 Oryctolagus cuniculus
3.5.4.3 0.00707
-
 guanine intestine enzyme at pH 7.5 Oryctolagus cuniculus
3.5.4.3 0.00726
-
 guanine brain enzyme at pH 7.5 Oryctolagus cuniculus
3.5.4.3 0.0073
-
 guanine intestine enzyme at pH 6.5 Oryctolagus cuniculus
3.5.4.3 0.00736
-
 guanine liver enzyme at pH 6.5 Oryctolagus cuniculus
3.5.4.3 0.00747
-
 guanine intestine enzyme at pH 6.0 Oryctolagus cuniculus
3.5.4.3 0.00762
-
 guanine liver enzyme at pH 7.5 Oryctolagus cuniculus
3.5.4.3 0.00819
-
 guanine brain enzyme at pH 6.5 Oryctolagus cuniculus
3.5.4.3 0.0105
-
 guanine at pH 8.2 Oryctolagus cuniculus
3.5.4.3 0.0125
-
 guanine
-
 Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.3 48000
-
 2 * 48000, SDS-PAGE Bos taurus
3.5.4.3 49500
-
 2 * 49500, SDS-PAGE Oryctolagus cuniculus
3.5.4.3 50000
-
 2 * 50000, SDS-PAGE Homo sapiens
3.5.4.3 50000
-
 2 * 50000, SDS-PAGE Oryctolagus cuniculus
3.5.4.3 50000
-
 2 * 50000, SDS-PAGE Sus sp.
3.5.4.3 52000
-
 2 * 52000, brain and liver enzymes, SDS-PAGE Bos taurus
3.5.4.3 52000
-
 2 * 52000, brain and liver enzymes, SDS-PAGE Oryctolagus cuniculus
3.5.4.3 56000
-
 liver enzyme, gel filtration Oryctolagus cuniculus
3.5.4.3 59000
-
 2 * 59000, SDS-PAGE Homo sapiens
3.5.4.3 92000
-
-
 Oryctolagus cuniculus
3.5.4.3 92200
-
 gel filtration, non denaturing PAGE Oryctolagus cuniculus
3.5.4.3 95000
-
-
 Bos taurus
3.5.4.3 95800
-
 liver enzyme, gel filtration Oryctolagus cuniculus
3.5.4.3 100000
-
-
 Homo sapiens
3.5.4.3 100000
-
-
 Oryctolagus cuniculus
3.5.4.3 105000
-
-
 Bos taurus
3.5.4.3 107800
-
 crosslinking Oryctolagus cuniculus
3.5.4.3 110000
-
-
 Sus sp.
3.5.4.3 110000
-
 liver, gel filtration Homo sapiens
3.5.4.3 110000
-
 liver, gel filtration Oryctolagus cuniculus
3.5.4.3 120000
-
-
 Homo sapiens
3.5.4.3 120000
-
 brain and liver enzymes Cavia porcellus
3.5.4.3 200000
-
 brain enzyme Homo sapiens
3.5.4.3 240000
-
 gel filtration without thiol present, enzyme aggregation Homo sapiens
3.5.4.3 240000
-
 gel filtration without thiol present, enzyme aggregation Sus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.3 guanine + H2O Cavia porcellus
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Homo sapiens
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Bos taurus
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Oryctolagus cuniculus
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Ovis ammon
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Sus sp.
-
 xanthine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.3 Bos taurus
-
 cow
-
3.5.4.3 Cavia porcellus
-
 guinea pig
-
3.5.4.3 Homo sapiens
-
 human
-
3.5.4.3 Oryctolagus cuniculus
-
 rabbit
-
3.5.4.3 Ovis ammon
-
 sheep
-
3.5.4.3 Sus sp.
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.3
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.3 brain
-
 Cavia porcellus
-
3.5.4.3 brain
-
 Homo sapiens
-
3.5.4.3 brain
-
 Oryctolagus cuniculus
-
3.5.4.3 brain
-
 Sus sp.
-
3.5.4.3 intestine
-
 Oryctolagus cuniculus
-
3.5.4.3 liver
-
 Cavia porcellus
-
3.5.4.3 liver
-
 Homo sapiens
-
3.5.4.3 liver
-
 Bos taurus
-
3.5.4.3 liver
-
 Oryctolagus cuniculus
-
3.5.4.3 pancreas
-
 Homo sapiens
-
3.5.4.3 pancreas
-
 Oryctolagus cuniculus
-
3.5.4.3 spleen
-
 Homo sapiens
-
3.5.4.3 spleen
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.3 11.2
-
 intestine enzyme, purification at 0-4°C Oryctolagus cuniculus
3.5.4.3 16.8
-
 brain enzyme, purification at room temperature Oryctolagus cuniculus
3.5.4.3 17.2
-
 liver enzyme, purification at room temperature Oryctolagus cuniculus
3.5.4.3 19.3
-
 liver enzyme, purification at 0-4°C Oryctolagus cuniculus
3.5.4.3 22.4
-
 brain enzyme, purification at 0-4°C Oryctolagus cuniculus
3.5.4.3 25.4
-
 intestine enzyme, purification at room temperature Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
3.5.4.3 purified enzyme stabilized by storage in 30-40% glycerol Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.3 guanine + H2O
-
 Cavia porcellus xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Homo sapiens xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Bos taurus xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Oryctolagus cuniculus xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Ovis ammon xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Sus sp. xanthine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.3 dimer 2 * 50000, SDS-PAGE Homo sapiens
3.5.4.3 dimer 2 * 50000, SDS-PAGE Oryctolagus cuniculus
3.5.4.3 dimer 2 * 50000, SDS-PAGE Sus sp.
3.5.4.3 dimer 2 * 59000, SDS-PAGE Homo sapiens
3.5.4.3 dimer 2 * 48000, SDS-PAGE Bos taurus
3.5.4.3 dimer 2 * 52000, brain and liver enzymes, SDS-PAGE Bos taurus
3.5.4.3 dimer 2 * 52000, brain and liver enzymes, SDS-PAGE Oryctolagus cuniculus
3.5.4.3 dimer 2 * 49500, SDS-PAGE Oryctolagus cuniculus
3.5.4.3 monomer 1 * 56000 Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.3 6.8
-
-
 Oryctolagus cuniculus
3.5.4.3 7.7
-
-
 Oryctolagus cuniculus
3.5.4.3 8
-
-
 Homo sapiens
3.5.4.3 8
-
-
 Oryctolagus cuniculus
3.5.4.3 8 8.5
-
 Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.3 5 8.5 activity relatively low at pH 5.1-5.5, increase in activity above pH 6.0 until pH 8.5, above pH 8.5 activity starts to drop off Oryctolagus cuniculus