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Literature summary extracted from

  • Yamaguchi, I.; Shibata, H.; Seto, H.; Misato, T.
    Isolation and purification of blasticidin S deaminase from Aspergillus terreus (1975), J. Antibiot., 28, 7-14.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.23 2-mercaptoethanol protecting enzyme Aspergillus terreus
3.5.4.23 blasticidin S produces far more enzyme when grown in presence Aspergillus terreus
3.5.4.23 Gougerotin enzyme induced by Aspergillus terreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.23 CuCl2
-
 Aspergillus terreus
3.5.4.23 HgCl2
-
 Aspergillus terreus
3.5.4.23 p-chloromercuribenzoate
-
 Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.23 29600
-
 sedimentation analysis Aspergillus terreus
3.5.4.23 30000
-
 SDS-PAGE Aspergillus terreus
3.5.4.23 46000
-
 gel filtration Aspergillus terreus
3.5.4.23 55000
-
 gel filtration, buffer without sodium chloride Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.23 blasticidin S + H2O Aspergillus flavus
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus terreus
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus ochraceus
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus fumigatus
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus ustus
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus flavipes
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus flavipes NHL 5013
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus ustus 1004
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus fumigatus NHL 5009
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus ochraceus 67-MI-87
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus terreus NHL 5037
-
 deaminohydroxyblasticidin S + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.23 Aspergillus flavipes
-
 NHL 5013
-
3.5.4.23 Aspergillus flavipes NHL 5013
-
 NHL 5013
-
3.5.4.23 Aspergillus flavus
-
 ATCC 9643
-
3.5.4.23 Aspergillus fumigatus
-
 NHL 5009
-
3.5.4.23 Aspergillus fumigatus NHL 5009
-
 NHL 5009
-
3.5.4.23 Aspergillus ochraceus
-
 67-MI-87
-
3.5.4.23 Aspergillus ochraceus 67-MI-87
-
 67-MI-87
-
3.5.4.23 Aspergillus terreus
-
 NHL 5037
-
3.5.4.23 Aspergillus terreus NHL 5037
-
 NHL 5037
-
3.5.4.23 Aspergillus ustus
-
 1004
-
3.5.4.23 Aspergillus ustus 1004
-
 1004
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.23
-
 Aspergillus terreus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.23 220.5
-
-
 Aspergillus terreus

Storage Stability

EC Number Storage Stability Organism
3.5.4.23 -20°C, 50% glycerol 5 mM mercaptoethanol, no appreciable loss of activity after 3 months Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.23 acetylblasticidin S + H2O
-
 Aspergillus terreus acetyldeaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 acetylblasticidin S + H2O
-
 Aspergillus terreus NHL 5037 acetyldeaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus flavus deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus terreus deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus ochraceus deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus fumigatus deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus ustus deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus flavipes deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus flavipes NHL 5013 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus ustus 1004 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus fumigatus NHL 5009 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus ochraceus 67-MI-87 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus terreus NHL 5037 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 cytidine + H2O only slightly deaminated Aspergillus terreus ?
-
 ?
3.5.4.23 cytomycin + H2O
-
 Aspergillus terreus ?
-
 ?
3.5.4.23 cytomycin + H2O
-
 Aspergillus terreus NHL 5037 ?
-
 ?
3.5.4.23 deoxycytidine + H2O only slightly deaminated Aspergillus terreus ?
-
 ?
3.5.4.23 gougerotin + H2O only slightly deaminated Aspergillus terreus ?
-
 ?
3.5.4.23 gougerotin + H2O only slightly deaminated Aspergillus terreus NHL 5037 ?
-
 ?
3.5.4.23 additional information cytosine, cytosinine, CMP, d-CMP, adenine, adenosine, AMP, guanine, guanosine, GMP or other purine bases or their nucleosides are no substrates Aspergillus terreus ?
-
 ?
3.5.4.23 additional information cytosine, cytosinine, CMP, d-CMP, adenine, adenosine, AMP, guanine, guanosine, GMP or other purine bases or their nucleosides are no substrates Aspergillus terreus NHL 5037 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.23 Bc S deaminase
-
 Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.23 60 70
-
 Aspergillus terreus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.4.23 20 80
-
 Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.23 60 80 reaction proceeds most rapidly at 60-70°C, occurs only during a short initial period at 80°C Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.23 10
-
 Tris-HCl, glycinate or bicarbonate buffer Aspergillus terreus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.23 5 12
-
 Aspergillus terreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.4.23 4.5
-
 rapid inactivation below Aspergillus terreus