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Literature summary extracted from
- Cytosine and cytidine deaminase from yeast (1978), Methods Enzymol., 51, 394-401.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.1 | CDP | - |
Saccharomyces cerevisiae |  |
| 3.5.4.1 | CMP | - |
Saccharomyces cerevisiae | |
| 3.5.4.1 | CTP | - |
Saccharomyces cerevisiae | |
| 3.5.4.1 | cytidine | - |
Saccharomyces cerevisiae | |
| 3.5.4.1 | dTTP | - |
Saccharomyces cerevisiae | |
| 3.5.4.1 | GDP | - |
Saccharomyces cerevisiae | |
| 3.5.4.1 | GMP | - |
Saccharomyces cerevisiae | |
| 3.5.4.1 | GTP | - |
Saccharomyces cerevisiae | |
| 3.5.4.1 | guanosine | - |
Saccharomyces cerevisiae | |
| 3.5.4.1 | thymidine | - |
Saccharomyces cerevisiae | |
| 3.5.4.5 | CDP | - |
Saccharomyces cerevisiae | |
| 3.5.4.5 | CMP | 0.300 mM 50% inhibition, 0.750 mM 90% inhibition | Saccharomyces cerevisiae | |
| 3.5.4.5 | CTP | - |
Saccharomyces cerevisiae | |
| 3.5.4.5 | cytidine | strong substrate inhibition above 0.5 mM | Saccharomyces cerevisiae | |
| 3.5.4.5 | dTMP | 0.400 mM 50% inhibition | Saccharomyces cerevisiae | |
| 3.5.4.5 | dTTP | - |
Mus musculus | |
| 3.5.4.5 | dTTP | 0.400 mM 50% inhibition | Saccharomyces cerevisiae | |
| 3.5.4.5 | GTP | 0.750 mM 85% inhibition | Saccharomyces cerevisiae | |
| 3.5.4.5 | p-mercuribenzoate | highly sensitive | Mus musculus | |
| 3.5.4.5 | p-mercuribenzoate | highly sensitive | Saccharomyces cerevisiae | |
| 3.5.4.5 | UTP | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.4.1 | 2.5 | - |
cytosine | - |
Saccharomyces cerevisiae | |
| 3.5.4.1 | 2.5 | - |
5-Methylcytosine | - |
Saccharomyces cerevisiae | |
| 3.5.4.5 | 0.091 | - |
2-deoxycytidine | - |
Saccharomyces cerevisiae | |
| 3.5.4.5 | 0.25 | - |
cytidine | - |
Escherichia coli | |
| 3.5.4.5 | 0.25 | - |
cytidine | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 34000 | - |
- |
Saccharomyces cerevisiae |
| 3.5.4.5 | 57000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.1 | cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | Mus musculus | - |
uridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | Escherichia coli | - |
uridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | Saccharomyces cerevisiae | - |
uridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | Mus musculus BALB/c | - |
uridine + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.1 | Saccharomyces cerevisiae | - |
- |
- |
| 3.5.4.5 | Escherichia coli | - |
- |
- |
| 3.5.4.5 | Mus musculus | - |
mouse | - |
| 3.5.4.5 | Mus musculus | - |
BALB/c | - |
| 3.5.4.5 | Mus musculus BALB/c | - |
BALB/c | - |
| 3.5.4.5 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.1 | - |
Saccharomyces cerevisiae |
| 3.5.4.5 | - |
Saccharomyces cerevisiae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.4.5 | kidney | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.5 | 3.128 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.4.1 | 4°C, stable in the pH-range 5-9 for at least 48 h | Saccharomyces cerevisiae |
| 3.5.4.5 | -20°C, shows no appreciable loss of activity within 1 month | Saccharomyces cerevisiae |
| 3.5.4.5 | 4°C, stable for some hours at pH 5.5-9.5 | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.1 | 5-methylcytosine + H2O | - |
Saccharomyces cerevisiae | thymine + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
| 3.5.4.5 | 2'-deoxycytidine + H2O | - |
Mus musculus | 2'-deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | 2'-deoxycytidine + H2O | - |
Escherichia coli | 2'-deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | 2'-deoxycytidine + H2O | - |
Saccharomyces cerevisiae | 2'-deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | 2'-deoxycytidine + H2O | - |
Mus musculus BALB/c | 2'-deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | - |
Mus musculus | uridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | - |
Escherichia coli | uridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | - |
Saccharomyces cerevisiae | uridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | - |
Mus musculus BALB/c | uridine + NH3 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.4.5 | yeast cytidine deaminase | - |
Saccharomyces cerevisiae |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.5 | 40 | 62 | heat treatment for 5 min at 50-60°C completely abolishes CMP and substrate inhibition | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 6.5 | - |
- |
Saccharomyces cerevisiae |
| 3.5.4.5 | 7.2 | - |
- |
Mus musculus |
| 3.5.4.5 | 7.2 | - |
- |
Saccharomyces cerevisiae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.5 | 5.5 | 9.5 | stable for some hours at 4°C, rapid inactivation and precipitation of the enzyme occurs at pH lower 5 | Saccharomyces cerevisiae |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.4.5 | 0.3 | - |
CMP | - |
Saccharomyces cerevisiae | |
| 3.5.4.5 | 0.4 | - |
dTMP | - |
Saccharomyces cerevisiae | |
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