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Literature summary extracted from

  • Yu, T.; Sakai, T.; Omata, S.
    Kinetic properties of cytosine deaminase from Serratia marcescens (1976), Agric. Biol. Chem., 40, 543-549.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.1 ATP
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 ATP 10.0 mM, relative activity 154% Serratia marcescens
3.5.4.1 dAMP 10.0 mM, relative activity 154% Serratia marcescens
3.5.4.1 GDP 10.0 mM, relative activity 176% Serratia marcescens
3.5.4.1 GTP
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 GTP 10.0 mM, relative activity 171% Serratia marcescens
3.5.4.1 IDP 10.0 mM, relative activity 152% Serratia marcescens
3.5.4.1 ITP
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 ITP 10.0 mM, relative activity 155% Serratia marcescens
3.5.4.1 L-amino acids
-
 Escherichia coli
3.5.4.1 L-amino acids
-
 Serratia marcescens
3.5.4.1 L-amino acids
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-asparagine
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-asparagine 10.0 mM, relative activity 150% Serratia marcescens
3.5.4.1 L-aspartic acid
-
 Escherichia coli
3.5.4.1 L-aspartic acid
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-aspartic acid 10.0 mM, relative activity 144% Serratia marcescens
3.5.4.1 L-cysteine 10.0 mM, relative activity 160% Serratia marcescens
3.5.4.1 L-Glutamic acid
-
 Escherichia coli
3.5.4.1 L-Glutamic acid
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-Glutamic acid 10.0 mM, relative activity 138% Serratia marcescens
3.5.4.1 L-glutamine
-
 Escherichia coli
3.5.4.1 L-glutamine
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-glutamine 10.0 mM, relative activity 143% Serratia marcescens
3.5.4.1 L-histidine
-
 Escherichia coli
3.5.4.1 L-histidine
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-histidine 10.0 mM, relative activity 160% Serratia marcescens
3.5.4.1 L-hydroxyproline
-
 Escherichia coli
3.5.4.1 L-hydroxyproline
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-hydroxyproline 10.0 mM, relative activity 150% Serratia marcescens
3.5.4.1 L-methionine
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-methionine 10.0 mM, relative activity 150% Serratia marcescens
3.5.4.1 phosphate
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 phosphate 1.0 mM, relative activity 170% Serratia marcescens
3.5.4.1 sulfate 1.0 mM, relative activity 110% Serratia marcescens
3.5.4.1 sulfite 1.0 mM, relative activity 105% Serratia marcescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.1 Cd2+
-
 Serratia marcescens
3.5.4.1 Co2+
-
 Serratia marcescens
3.5.4.1 Cu2+
-
 Escherichia coli
3.5.4.1 Cu2+
-
 Serratia marcescens
3.5.4.1 deoxyguanosine
-
 Serratia marcescens
3.5.4.1 dGMP
-
 Serratia marcescens
3.5.4.1 Hg2+
-
 Escherichia coli
3.5.4.1 Hg2+
-
 Serratia marcescens
3.5.4.1 Ni2+
-
 Serratia marcescens
3.5.4.1 Zn2+
-
 Escherichia coli
3.5.4.1 Zn2+
-
 Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.1 3.4
-
 cytosine
-
 Serratia marcescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.1 BaCl2
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 BaCl2 1.0 mM, relative activity 110% Serratia marcescens
3.5.4.1 FeCl3
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 FeCl3 1.0 mM, relative activity 115% Serratia marcescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.1 cytosine + H2O Escherichia coli
-
 uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O Saccharomyces cerevisiae
-
 uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O Serratia marcescens
-
 uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O Pseudomonas chlororaphis subsp. aureofaciens
-
 uracil + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Escherichia coli
-
-
-
3.5.4.1 Pseudomonas chlororaphis subsp. aureofaciens
-
-
-
3.5.4.1 Saccharomyces cerevisiae
-
 baker's yeast
-
3.5.4.1 Saccharomyces cerevisiae
-
 type Y
-
3.5.4.1 Serratia marcescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.1
-
 Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 5-methylcytosine + H2O
-
 Saccharomyces cerevisiae thymine + NH3
-
 ?
3.5.4.1 5-methylcytosine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens thymine + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Escherichia coli uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Saccharomyces cerevisiae uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Serratia marcescens uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens uracil + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.1 cytosine deaminase Y
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.1 45
-
-
 Serratia marcescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.4.1 30 70
-
 Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.1 7
-
-
 Saccharomyces cerevisiae
3.5.4.1 9.5
-
 with cytosine as substrate Serratia marcescens