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Literature summary extracted from

  • Yorifuji, T.; Kato, M.; Kobayashi, T.; Ozaki, S.; Ueno, S.
    4-Guanidinobutyrate amidinohydrolase from Pseudomonas sp. ATCC 14676: Purification to homogeneity and properties (1980), Agric. Biol. Chem., 44, 1127-1134.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.3.7
-
 Pseudomonas sp.

General Stability

EC Number General Stability Organism
3.5.3.7 relatively unstable in absence of Mn2+ Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.7 2-nitrobenzoic acid slight inhibition Pseudomonas sp.
3.5.3.7 EDTA
-
 Pseudomonas sp.
3.5.3.7 n-Butyrate competitive inhibition Pseudomonas sp.
3.5.3.7 N-ethylmaleimide slight inhibition Pseudomonas sp.
3.5.3.7 p-chloromercuribenzoate reactivation by incubation with 2-mercaptoethanol Pseudomonas sp.
3.5.3.7 propionate competitive inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.7 33
-
 4-Guanidinobutyrate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.7 Ni2+ slight activation Pseudomonas sp.
3.5.3.7 Zn2+ slight activation Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.7 180000 186000 gel filtration, disc gel electrophoresis Pseudomonas sp.
3.5.3.7 185000
-
 disc gel electrophoresis Pseudomonas sp.
3.5.3.7 240000
-
-
 Flavobacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas fluorescens
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas sp.
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Flavobacterium sp.
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas sp. ATCC 14676
-
 4-Aminobutyrate + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.7 Flavobacterium sp.
-
-
-
3.5.3.7 Pseudomonas fluorescens
-
-
-
3.5.3.7 Pseudomonas sp.
-
-
-
3.5.3.7 Pseudomonas sp. ATCC 14676
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.7
-
 Pseudomonas fluorescens
3.5.3.7
-
 Pseudomonas sp.
3.5.3.7
-
 Flavobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.7 252
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas fluorescens 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas sp. 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Flavobacterium sp. 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas sp. ATCC 14676 4-Aminobutyrate + urea
-
 ?
3.5.3.7 5-guanidinovalerate + H2O
-
 Pseudomonas fluorescens 5-aminopentanoate + urea
-
 ?
3.5.3.7 5-guanidinovalerate + H2O
-
 Pseudomonas sp. 5-aminopentanoate + urea
-
 ?
3.5.3.7 5-guanidinovalerate + H2O
-
 Pseudomonas sp. ATCC 14676 5-aminopentanoate + urea
-
 ?
3.5.3.7 6-guanidinocaproate + H2O
-
 Pseudomonas fluorescens 6-aminohexanoate + urea
-
 ?
3.5.3.7 6-guanidinocaproate + H2O
-
 Pseudomonas sp. 6-aminohexanoate + urea
-
 ?
3.5.3.7 6-guanidinocaproate + H2O
-
 Pseudomonas sp. ATCC 14676 6-aminohexanoate + urea
-
 ?
3.5.3.7 additional information guanidinoacetate, 3-guanidinopropionate, 3-guanidino-n-butyrate, taurocyamine, creatine, N-amidinoalanine, L-arginine, D-arginine, DL-N-acetyl-arginine, canavanine, agmatine, methylguanidine and guanylurea are no substrates Pseudomonas sp. ?
-
 ?
3.5.3.7 additional information guanidinoacetate, 3-guanidinopropionate, 3-guanidino-n-butyrate, taurocyamine, creatine, N-amidinoalanine, L-arginine, D-arginine, DL-N-acetyl-arginine, canavanine, agmatine, methylguanidine and guanylurea are no substrates Pseudomonas sp. ATCC 14676 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.7 hexamer 6 * 33000-36000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.7 70
-
 rather heat stable, no detectable loss of activity up to Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.7 10.2
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.7 7.5 8.5 most stable at, at pH higher than 9.0 or lower than 6.5 potassium phosphate 50 mM and sodium chloride 0.3 M yields a stabilizing effect at pH 7.5-10.0 Pseudomonas sp.