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Literature summary extracted from

  • Shoeb, S.M.; Kaneoke, M.; Shimizu, E.; Yorifuji, T.
    Diguanidinobutanase of Pseudomonas putida and enzymatic preparation of agmatine from 1,4-diguanidinobutanate (1995), Appl. Microbiol. Biotechnol., 44, 94-99.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.3.20 synthesis preparation of pure agmatine sulfate Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.20 1,10-Diaminodecane 41% inhibition at concentration of 3.0 mM Pseudomonas putida
3.5.3.20 1,7-Diaminoheptane 22% inhibition at concentration of 5.0 mM Pseudomonas putida
3.5.3.20 1,8-diaminooctane 34% inhibition at concentration of 3.0 mM Pseudomonas putida
3.5.3.20 1,9-diaminononane 38% inhibition at concentration of 3.0 mM Pseudomonas putida
3.5.3.20 1-amino-5-guanidinopentane 29% inhibition at concentration of 5.0 mM Pseudomonas putida
3.5.3.20 5-Guanidinovalerate 10% inhibition at concentration of 5.0 mM Pseudomonas putida
3.5.3.20 agmatine 28% inhibition at concentration of 5.0 mM Pseudomonas putida
3.5.3.20 DTNB 11% inhibition at concentration of 5.0 mM Pseudomonas putida
3.5.3.20 N-carbamoylagmatine 62% inhibition at concentration of 5.0 mM Pseudomonas putida
3.5.3.20 p-chloromercuribenzoate complete loss of activity at concentration of 5.0 mM, 30% reactivation by incubation with 10.0 mM 2-mercaptoethanol Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.20 0.23
-
 1,8-Diguanidinooctane
-
 Pseudomonas putida
3.5.3.20 0.24
-
 1,7-Diguanidinoheptane
-
 Pseudomonas putida
3.5.3.20 0.33
-
 1,6-Diguanidinohexane
-
 Pseudomonas putida
3.5.3.20 0.5
-
 1,5-Diguanidinopentane
-
 Pseudomonas putida
3.5.3.20 0.65
-
 1,4-Diguanidinobutane
-
 Pseudomonas putida
3.5.3.20 0.91
-
 1,3-diguanidinopropane
-
 Pseudomonas putida
3.5.3.20 1
-
 1-amino-7-guanidinoheptane
-
 Pseudomonas putida
3.5.3.20 1.3
-
 1-amino-6-guanidinohexane
-
 Pseudomonas putida
3.5.3.20 2.5
-
 1-amino-5-guanidinopentane
-
 Pseudomonas putida
3.5.3.20 3
-
 agmatine
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.20 Ca2+ inert Pseudomonas putida
3.5.3.20 Co2+ required for full activity Pseudomonas putida
3.5.3.20 Mg2+ inert Pseudomonas putida
3.5.3.20 Mn2+ required for full activity, no inactivation by incubation with 10 mM EDTA Pseudomonas putida
3.5.3.20 Mn2+ two ions per subunit Pseudomonas putida
3.5.3.20 Ni2+ required for full activity Pseudomonas putida
3.5.3.20 Zn2+ inert Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.20 170000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.20 1,4-diguanidinobutane + H2O Pseudomonas putida
-
 agmatine + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.20 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.20 full purification Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
3.5.3.20 pH 7.0-8.0 Pseudomonas putida
3.5.3.20 pH 7.0-9.0 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.20 1,3-diguanidinopropane + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.3.20 1,4-diguanidinobutane + H2O
-
 Pseudomonas putida agmatine + urea
-
 ?
3.5.3.20 1,5-diguanidinopentane + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.3.20 1,6-diguanidinohexane + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.3.20 1,7-diguanidinoheptane + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.3.20 1,8-diguanidinooctane + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.3.20 1-amino-5-guanidinopentane + H2O
-
 Pseudomonas putida 1,5-diaminopentane + urea
-
 ?
3.5.3.20 1-amino-6-guanidinohexane + H2O
-
 Pseudomonas putida 1,6-diaminohexane + urea
-
 ?
3.5.3.20 1-amino-7-guanidinoheptane + H2O
-
 Pseudomonas putida 1,7-diaminoheptane + urea
-
 ?
3.5.3.20 agmatine + H2O low activity towards agmatine Pseudomonas putida putrescine + urea
-
 ?
3.5.3.20 diguanidinoalkanoate + H2O utilization of alkanoates with 3-10 methylen groups Pseudomonas putida aminoalkylguanidine + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.20 tetramer 4*38000 Da, SDS-PAGE Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.20 30
-
 no loss of activity within 2 h in presence of 10 mM EDTA Pseudomonas putida
3.5.3.20 40
-
 stable on heating in presence of Mn2+ Pseudomonas putida
3.5.3.20 60
-
 complete inactivation in presence and absence of Mn2+ Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.20 1330
-
 1,4-Diguanidinobutane per subunit Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.20 10 11 in sodium carbonate and piperazine hydrochloric buffers Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.20 7 9 stable in TRIS-HCl Pseudomonas putida
3.5.3.20 7 8 stable in potassium phosphate Pseudomonas putida
3.5.3.20 10
-
 stable in piperazine hydrochloride Pseudomonas putida