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Literature summary extracted from

  • Yorifuji, T.; Kaneoke, M.; Shimizu, E.; Shiota, K.; Matsuo, R.
    Degradation of alpha,omega-diguanidinoalkanoates and a novel enzyme, diguanidinobutane amidohydrolase in Pseudomonas putida (1989), Agric. Biol. Chem., 53, 3003-3009.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.20 0.7
-
 1,4-Diguanidinobutane
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.20 Co2+ required for full activity Pseudomonas putida
3.5.3.20 Mn2+ required for full activity, no inactivation by incubation with 10 mM EDTA Pseudomonas putida
3.5.3.20 Ni2+ required for full activity Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.20 1,4-diguanidinobutane + H2O Pseudomonas putida
-
 agmatine + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.20 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.20 partial purification Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.20 1,4-diguanidinobutane + H2O
-
 Pseudomonas putida agmatine + urea
-
 ?
3.5.3.20 agmatine + H2O low activity towards agmatine Pseudomonas putida putrescine + urea
-
 ?
3.5.3.20 diguanidinoalkanoate + H2O utilization of alkanoates with 3-10 methylen groups Pseudomonas putida aminoalkylguanidine + urea
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.20 40
-
 stable on heating for 15 min Pseudomonas putida
3.5.3.20 50
-
 unstable on heating for 15 min Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.20 10 11
-
 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.20 7 10 most stable Pseudomonas putida