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Literature summary extracted from

  • Shirokane, Y.; Nakayima, M.
    Purification and some properties of guanidinoacetate amidinohydrolase produced by Corynebacterium sp. (1986), J. Ferment. Technol., 64, 29-36.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.3.2 2-mercaptoethanol 1 mM, activity 102% Corynebacterium sp.

Application

EC Number Application Comment Organism
3.5.3.2 medicine enzymic determination of guanidinoacetic acid in urine, guanidinoacetic acid decreases in patients with kidney disease or patients who have undergone kidney transplantation, low amount of guanidinoacetic acid in urine is an indicator of renal dysfunction Corynebacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.2 1,10-phenanthroline
-
 Corynebacterium sp.
3.5.3.2 1,10-phenanthroline
-
 Flavobacterium sp.
3.5.3.2 8-hydroxyquinoline
-
 Corynebacterium sp.
3.5.3.2 Fe2+ 1 mM, plus 0.1 mM Mn2+ partial inhibition, relative activity 40% Corynebacterium sp.
3.5.3.2 glycine competitive inhibition Corynebacterium sp.
3.5.3.2 Hg2+
-
 Corynebacterium sp.
3.5.3.2 Iodine
-
 Corynebacterium sp.
3.5.3.2 n-butylguanidine
-
 Corynebacterium sp.
3.5.3.2 n-Decylamine
-
 Corynebacterium sp.
3.5.3.2 n-dodecylamine
-
 Corynebacterium sp.
3.5.3.2 n-octylamine
-
 Corynebacterium sp.
3.5.3.2 n-propylguanidine
-
 Corynebacterium sp.
3.5.3.2 p-chloromercuribenzoate
-
 Corynebacterium sp.
3.5.3.2 p-chloromercuribenzoate
-
 Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.2 16
-
 Guanidinoacetate
-
 Corynebacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.2 Mn2+
-
 Corynebacterium sp.
3.5.3.2 Ni2+
-
 Corynebacterium sp.
3.5.3.2 Zn2+ restores activity of 1,10-phenanthroline treated enzyme Corynebacterium sp.
3.5.3.2 Zn2+ restores activity of 1,10-phenanthroline treated enzyme Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.2 38000
-
 4 * 38000, SDS-PAGE Corynebacterium sp.
3.5.3.2 150000
-
 gel filtration Corynebacterium sp.
3.5.3.2 281000
-
-
 Flavobacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.2 guanidinoacetate + H2O Pseudomonas putida
-
 glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O Corynebacterium sp.
-
 glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O Penicillium roqueforti
-
 glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O Flavobacterium sp.
-
 glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O Corynebacterium sp. N-19-1
-
 glycine + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.2 Corynebacterium sp.
-
 N-19-1
-
3.5.3.2 Corynebacterium sp. N-19-1
-
 N-19-1
-
3.5.3.2 Flavobacterium sp.
-
 F. sp. GE-1
-
3.5.3.2 Penicillium roqueforti
-
-
-
3.5.3.2 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.2
-
 Corynebacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.2 25.8
-
-
 Corynebacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.2 3-guanidinopropionate + H2O
-
 Corynebacterium sp. 3-aminopapanoate + urea
-
 ?
3.5.3.2 3-guanidinopropionate + H2O
-
 Flavobacterium sp. 3-aminopapanoate + urea
-
 ?
3.5.3.2 3-guanidinopropionate + H2O
-
 Corynebacterium sp. N-19-1 3-aminopapanoate + urea
-
 ?
3.5.3.2 4-guanidinobutyrate + H2O
-
 Pseudomonas putida 4-aminobutanoate + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O
-
 Pseudomonas putida glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O
-
 Corynebacterium sp. glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O
-
 Penicillium roqueforti glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O
-
 Flavobacterium sp. glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O
-
 Corynebacterium sp. N-19-1 glycine + urea
-
 ?
3.5.3.2 additional information
-
 Flavobacterium sp. ?
-
 ?
3.5.3.2 additional information guanidine, methylguanidine, ethylguanidine, n-propylguanidine, L-arginine, N-acetyl-L-arginine, L-argininate, agmatine, creatine, creatinine and guanidinosuccinate are no substrates Corynebacterium sp. ?
-
 ?
3.5.3.2 additional information guanidine, methylguanidine, ethylguanidine, n-propylguanidine, L-arginine, N-acetyl-L-arginine, L-argininate, agmatine, creatine, creatinine and guanidinosuccinate are no substrates Corynebacterium sp. N-19-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.2 tetramer
-
 Flavobacterium sp.
3.5.3.2 tetramer 4 * 38000, SDS-PAGE Corynebacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.2 55
-
-
 Corynebacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.2 9 9.5
-
 Corynebacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.2 6 10.5 stable in this range at 30°C for 1 h Corynebacterium sp.