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Literature summary extracted from

  • Yorifuji, T.; Furuyoshi, S.
    Mn2+ dependent amidinoaspartase in Pseudomonas putida (1986), Agric. Biol. Chem., 50, 1327-1328.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.3.14 medicine test for N-amidino-L-aspartate in the urine of uremic patients Pseudomonas chlororaphis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.14 EDTA
-
 Pseudomonas chlororaphis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.14 0.0094
-
 N-amidino-L-aspartate C-32, activation with Mn2+ Pseudomonas putida
3.5.3.14 0.21
-
 N-amidino-L-aspartate C-32 , activation with Co2+ Pseudomonas putida
3.5.3.14 10.3
-
 N-amidino-L-aspartate C-32 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.14 Co2+
-
 Pseudomonas chlororaphis
3.5.3.14 Co2+ C-32, 27% of the activation with Mn2+ Pseudomonas putida
3.5.3.14 Mn2+ C-32, specifically activation Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.14 39800
-
 5 * 39800, C-32, SDS-PAGE Pseudomonas putida
3.5.3.14 240000
-
 C-32, gel filtration Pseudomonas putida
3.5.3.14 300000
-
-
 Pseudomonas chlororaphis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.14 N-amidino-L-aspartic acid Pseudomonas putida
-
 L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-aspartic acid Pseudomonas chlororaphis
-
 L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-aspartic acid Pseudomonas putida C-32
-
 L-aspartic acid + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.14 no activity in Pseudomonas chlororaphis
-
-
-
3.5.3.14 no activity in Pseudomonas chlororaphis ATCC 9446
-
-
-
3.5.3.14 Pseudomonas chlororaphis
-
 fluorescent Pseudomonas
-
3.5.3.14 Pseudomonas putida
-
 C-32
-
3.5.3.14 Pseudomonas putida C-32
-
 C-32
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.14
-
 Pseudomonas chlororaphis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.14 27.9
-
-
 Pseudomonas chlororaphis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.14 additional information enzyme is specific for the L-isomer Pseudomonas chlororaphis ?
-
 ?
3.5.3.14 additional information C-32, guanidinoacetate, 3-guanidinopropionate, 4-guanidinobutyrate, 5-guanidinovalerate, 6-guanidinocaproate, 2-guanidinoethylsulfate, taurocyamine, 4-guanidino-3-hydroxybutyrate, L-arginine, DL-N-acetylarginine, DL-2-guanidinoisobutyrate, N-amidinoalanine, methylguanidine, guanidine and creatine are no substrates Pseudomonas putida ?
-
 ?
3.5.3.14 additional information C-32, guanidinoacetate, 3-guanidinopropionate, 4-guanidinobutyrate, 5-guanidinovalerate, 6-guanidinocaproate, 2-guanidinoethylsulfate, taurocyamine, 4-guanidino-3-hydroxybutyrate, L-arginine, DL-N-acetylarginine, DL-2-guanidinoisobutyrate, N-amidinoalanine, methylguanidine, guanidine and creatine are no substrates Pseudomonas putida C-32 ?
-
 ?
3.5.3.14 N-amidino-L-aspartic acid
-
 Pseudomonas putida L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-aspartic acid
-
 Pseudomonas chlororaphis L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-aspartic acid
-
 Pseudomonas putida C-32 L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-aspartic acid + H2O
-
 Pseudomonas putida L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-aspartic acid + H2O
-
 Pseudomonas chlororaphis L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-aspartic acid + H2O
-
 Pseudomonas putida C-32 L-aspartic acid + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.14 pentamer 5 * 39800, C-32, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.5.3.14 Mn2+-dependent amidinoaspartase
-
 Pseudomonas chlororaphis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.14 9 10 C-32 Pseudomonas putida