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Literature summary extracted from

  • Hu, L.; Mulfinger, L.M.; Phillips, A.T.
    Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida (1987), J. Bacteriol., 169, 4696-4702.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.68 histidine induction and activation Pseudomonas putida
3.5.1.68 N-formyl-L-glutamate induction and activation Pseudomonas putida
3.5.1.68 Urocanate induction and activation Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.68 hutG , formylglutamate amidohydrolase gene, subcloned on a multicopy plasmid in Escherichia coli Pseudomonas putida
3.5.1.68 hutG, formylglutamate amidohydrolase gene, expressed in Escherichia coli RDP145 Pseudomonas putida
3.5.3.8 expression in Escherichia coli Pseudomonas putida
3.5.3.13 pMCl, entire group of hut genes from P. putida ATCC 12633 cloned into Escherichia coli , hutF encodes FIGLU iminohydrolase Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.68 Fe2+ slight inhibition above 0.1 mM, activation below 0.1 mM Pseudomonas putida
3.5.1.68 N-acetyl-L-glutamate kinetics, competitive inhibition, Ki: 6 mM Pseudomonas putida
3.5.1.68 N-acetyl-L-glutamine slight inhibition Pseudomonas putida
3.5.1.68 N-formyl-L-aspartate kinetics, competitive inhibition, Ki: 9 mM Pseudomonas putida
3.5.3.8 N-acetyl-L-glutamate competitive inhibitor Pseudomonas putida
3.5.3.8 N-formyl-L-aspartate competitive inhibitor Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.68 14
-
 N-formyl-L-glutamate
-
 Pseudomonas putida
3.5.3.8 14
-
 N-formimidoyl-L-glutamate
-
 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.68 cytosol present in soluble fractions Pseudomonas putida 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.68 Ca2+ no activation Pseudomonas putida
3.5.1.68 Co2+ activation, most effective, 0.8 mM: 4fold activation Pseudomonas putida
3.5.1.68 Divalent metal ions activation Pseudomonas putida
3.5.1.68 Fe2+ activation below 0.1 mM, slight inhibition above 0.1 mM Pseudomonas putida
3.5.1.68 Mg2+ no activation Pseudomonas putida
3.5.1.68 Mn2+ no activation Pseudomonas putida
3.5.1.68 additional information no activation by Cd2+ Pseudomonas putida
3.5.1.68 additional information no activation by Ni2+, Zn2+ Pseudomonas putida
3.5.3.8 Ca2+
-
 Pseudomonas putida
3.5.3.8 Cd2+
-
 Pseudomonas putida
3.5.3.8 Co2+
-
 Pseudomonas putida
3.5.3.8 Fe2+
-
 Pseudomonas putida
3.5.3.8 Mg2+
-
 Pseudomonas putida
3.5.3.8 Mn2+
-
 Pseudomonas putida
3.5.3.8 Ni2+
-
 Pseudomonas putida
3.5.3.8 Zn2+
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.68 50000
-
 1 * 50000, SDS-PAGE Pseudomonas putida
3.5.1.68 52000
-
 gel filtration Pseudomonas putida
3.5.3.8 50000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.68 N-formyl-L-glutamate + H2O Pseudomonas putida terminal reaction in the five-step pathway for histidine utilization formate + L-glutamate
-
 ?
3.5.1.68 N-formyl-L-glutamate + H2O Pseudomonas spp. terminal reaction in the five-step pathway for histidine utilization formate + L-glutamate
-
 ?
3.5.3.8 N-formimidoyl-L-glutamate + H2O Pseudomonas putida
-
 L-glutamate + formamide
-
 ?
3.5.3.13 N-formimino-L-glutamate + H2O Pseudomonas aeruginosa
-
 N-formyl-L-glutamate + NH3
-
 ?
3.5.3.13 N-formimino-L-glutamate + H2O Pseudomonas putida
-
 N-formyl-L-glutamate + NH3
-
 ?
3.5.3.13 N-formimino-L-glutamate + H2O Streptomyces coelicolor
-
 N-formyl-L-glutamate + NH3
-
 ?
3.5.3.13 N-formimino-L-glutamate + H2O Comamonas testosteroni
-
 N-formyl-L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.68 Pseudomonas putida
-
 wild-type strain PRS1, ATCC 12633 and several mutants
-
3.5.1.68 Pseudomonas spp.
-
 present in all species of Pseudomonas studied
-
3.5.3.8 Pseudomonas putida
-
-
-
3.5.3.13 Comamonas testosteroni
-
-
-
3.5.3.13 Pseudomonas aeruginosa
-
-
-
3.5.3.13 Pseudomonas putida
-
 ATCC 12633
-
3.5.3.13 Streptomyces coelicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.68 from recombinant Escherichia coli strain RDP145, pLH4 Pseudomonas putida
3.5.3.8
-
 Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.68 N-formyl-L-glutamate + H2O = formate + L-glutamate regulation, induction and catabolite repression of formylglutamate amidohydrolase Pseudomonas putida
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.68 additional information
-
-
 Pseudomonas putida
3.5.1.68 100
-
 in the presence of 5 mM substrate, N-formyl-L-glutamate, and 0.8 mM CoCl2, 30°C Pseudomonas putida
3.5.1.68 380
-
 calculated specific activity, with saturating substrate Pseudomonas putida
3.5.3.8 100
-
-
 Pseudomonas putida
3.5.3.13 0.003
-
 wild type PRS1, inducer N-formimino-L-glutamate Pseudomonas putida
3.5.3.13 0.005
-
 wild type PRS1, inducer N-formyl-L-glutamate Pseudomonas putida
3.5.3.13 0.005
-
 wild type PRS1 Pseudomonas putida
3.5.3.13 0.015
-
 wild type PRS1, inducer urocanate Pseudomonas putida
3.5.3.13 0.06
-
 strain PS15(hutU) Pseudomonas putida
3.5.3.13 0.066
-
 wild type PRS1, inducer histidine Pseudomonas putida
3.5.3.13 0.086
-
 strain LH13(hutC) Pseudomonas putida
3.5.3.13 0.09
-
 strain LH12(hutC) Pseudomonas putida
3.5.3.13 0.093
-
 strain LH11(hutC) Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
3.5.1.68 -70°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.68 additional information substrate specificity Pseudomonas putida ?
-
 ?
3.5.1.68 additional information little or no activity with N-acetyl-L-glutamate Pseudomonas putida ?
-
 ?
3.5.1.68 N-formyl-L-glutamate + H2O terminal reaction in the five-step pathway for histidine utilization Pseudomonas putida formate + L-glutamate
-
 ?
3.5.1.68 N-formyl-L-glutamate + H2O terminal reaction in the five-step pathway for histidine utilization Pseudomonas spp. formate + L-glutamate
-
 ?
3.5.3.8 N-formimidoyl-L-glutamate + H2O
-
 Pseudomonas putida L-glutamate + formamide
-
 ?
3.5.3.13 N-formimino-L-glutamate + H2O
-
 Pseudomonas aeruginosa N-formyl-L-glutamate + NH3
-
 ?
3.5.3.13 N-formimino-L-glutamate + H2O
-
 Pseudomonas putida N-formyl-L-glutamate + NH3
-
 ?
3.5.3.13 N-formimino-L-glutamate + H2O
-
 Streptomyces coelicolor N-formyl-L-glutamate + NH3
-
 ?
3.5.3.13 N-formimino-L-glutamate + H2O
-
 Comamonas testosteroni N-formyl-L-glutamate + NH3
-
 ?
3.5.3.13 N-formimino-L-glutamic acid + H2O
-
 Pseudomonas aeruginosa N-formyl-L-glutamic acid + NH3
-
 ?
3.5.3.13 N-formimino-L-glutamic acid + H2O
-
 Pseudomonas putida N-formyl-L-glutamic acid + NH3
-
 ?
3.5.3.13 N-formimino-L-glutamic acid + H2O
-
 Streptomyces coelicolor N-formyl-L-glutamic acid + NH3
-
 ?
3.5.3.13 N-formimino-L-glutamic acid + H2O
-
 Comamonas testosteroni N-formyl-L-glutamic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.68 monomer 1 * 50000, SDS-PAGE Pseudomonas putida
3.5.3.8 monomer 1 * 50000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.5.3.13 FIGLU-iminohydrolase
-
 Pseudomonas putida
3.5.3.13 FIGLUase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.68 30
-
 assay at Pseudomonas putida
3.5.3.8 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.68 7 8 maximally active at Pseudomonas putida
3.5.1.68 7.5
-
 assay at Pseudomonas putida
3.5.1.68 7.5
-
 100 mM potassium phosphate buffer Pseudomonas putida
3.5.3.8 7 8
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.68 6.6 8.5 more than 65% of maximal activity between pH 6.6 and 8.5 Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.68 6
-
 N-acetyl-L-glutamate kinetics, competitive inhibition Pseudomonas putida
3.5.1.68 9
-
 N-formyl-L-aspartate kinetics, competitive inhibition Pseudomonas putida