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Literature summary extracted from

  • Yanagisawa, H.; Suzuki, Y.
    Corn agmatine iminohydrolase. Purification and properties (1981), Plant Physiol., 67, 697-700.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.12 Ca2+
-
 Zea mays
3.5.3.12 cadaverine
-
 Arachis hypogaea
3.5.3.12 Co2+ 1 mM 55% inhibition Zea mays
3.5.3.12 Cu2+
-
 Arachis hypogaea
3.5.3.12 Cu2+ 1 mM 100% inhibition Zea mays
3.5.3.12 Fe2+
-
 Zea mays
3.5.3.12 Mg2+
-
 Zea mays
3.5.3.12 N,N'-diguanidinobutane
-
 Zea mays
3.5.3.12 Ni2+
-
 Zea mays
3.5.3.12 p-chloromercuribenzoate
-
 Arachis hypogaea
3.5.3.12 p-chloromercuribenzoate
-
 Zea mays
3.5.3.12 putrescine
-
 Arachis hypogaea
3.5.3.12 spermidine
-
 Arachis hypogaea
3.5.3.12 spermine
-
 Arachis hypogaea
3.5.3.12 tryptamine
-
 Arachis hypogaea
3.5.3.12 Zn2+
-
 Arachis hypogaea
3.5.3.12 Zn2+ 1 mM 85% inhibition Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.12 0.19
-
 agmatine
-
 Zea mays
3.5.3.12 0.76
-
 agmatine
-
 Arachis hypogaea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.3.12 cytosol
-
 Zea mays 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.12 43000
-
 2 * 43000, SDS-PAGE Zea mays
3.5.3.12 85000
-
 gel filtration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.12 agmatine + H2O Zea mays
-
 N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O Arachis hypogaea
-
 N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O Helianthus annuus
-
 N-carbamoylputrescine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.12 Arachis hypogaea
-
-
-
3.5.3.12 Helianthus annuus
-
-
-
3.5.3.12 Zea mays
-
 L. cv Goldencross Bantam T51
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.12
-
 Zea mays
3.5.3.12
-
 Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.12 cotyledon
-
 Arachis hypogaea
-
3.5.3.12 seedling
-
 Zea mays
-
3.5.3.12 seedling
-
 Helianthus annuus
-
3.5.3.12 shoot
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.12 876.3
-
-
 Zea mays

Storage Stability

EC Number Storage Stability Organism
3.5.3.12 4°C, purified enzyme lost 30% of the activity in 3 days Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.12 agmatine + H2O
-
 Zea mays N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O
-
 Arachis hypogaea N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O
-
 Helianthus annuus N-carbamoylputrescine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.12 dimer 2 * 43000, SDS-PAGE Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.12 60
-
-
 Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.12 50
-
 exposure for 10 min, decrease of 50% in the activity Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.12 6.5
-
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.12 6 7 activity rapidly declining below pH 6.0 and declining less rapidly above pH 7.0 Zea mays