Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ye, G.J.; Breslow, E.; Meister, A.
    The amino acid sequence of rat kidney 5-oxo-L-prolinase determined by cDNA cloning (1996), J. Biol. Chem., 271, 32293-32300.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.9 amino acid sequence determined by cDNA cloning, gene expressed in Escherichia coli Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.9 50000
-
component A 1 * 65500 + 1 * 50000, component B 1 * 80000, SDS-PAGE Pseudomonas putida
3.5.2.9 65500
-
component A 1 * 65500 + 1 * 50000, component B 1 * 80000, SDS-PAGE Pseudomonas putida
3.5.2.9 80000
-
component A 1 * 65500 + 1 * 50000, component B 1 * 80000, SDS-PAGE Pseudomonas putida
3.5.2.9 137800
-
calculated from amino acid sequence Rattus norvegicus
3.5.2.9 142000
-
2 * 142000, SDS-PAGE Rattus norvegicus
3.5.2.9 325000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.9 5-oxo-L-proline + H2O + ATP Pseudomonas putida
-
L-glutamate + ADP + phosphate
-
?
3.5.2.9 5-oxo-L-proline + H2O + ATP Rattus norvegicus
-
L-glutamate + ADP + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.9 Pseudomonas putida
-
-
-
3.5.2.9 Rattus norvegicus P97608 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.9
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.9 brain
-
Rattus norvegicus
-
3.5.2.9 cardiac muscle
-
Rattus norvegicus
-
3.5.2.9 kidney
-
Rattus norvegicus
-
3.5.2.9 liver
-
Rattus norvegicus
-
3.5.2.9 lung
-
Rattus norvegicus
-
3.5.2.9 skeletal muscle
-
Rattus norvegicus
-
3.5.2.9 spleen
-
Rattus norvegicus
-
3.5.2.9 testis
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
Pseudomonas putida L-glutamate + ADP + phosphate
-
?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
Rattus norvegicus L-glutamate + ADP + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.5.2.9 dimer 2 * 142000, SDS-PAGE Rattus norvegicus
3.5.2.9 polymer component A 1 * 65500 + 1 * 50000, component B 1 * 80000, SDS-PAGE Pseudomonas putida