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Literature summary extracted from

  • Van Der Werf, P.; Griffith, O.W.; Meister, A.
    5-Oxo-L-prolinase (L-pyroglutamate hydrolase) purification and catalytic properties (1975), J. Biol. Chem., 250, 6686-6692.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.9 iodoacetamide
-
 Rattus norvegicus
3.5.2.9 L-2-imidazolidone-4-carboxylate
-
 Mus musculus
3.5.2.9 L-2-imidazolidone-4-carboxylate
-
 Rattus norvegicus
3.5.2.9 L-dihydroorotic acid
-
 Mus musculus
3.5.2.9 L-dihydroorotic acid
-
 Rattus norvegicus
3.5.2.9 N-ethylmaleimide
-
 Rattus norvegicus
3.5.2.9 p-hydroxymercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.9 0.05
-
 5-oxoproline
-
 Rattus norvegicus
3.5.2.9 0.17
-
 ATP
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.9 K+ requires monovalent cation, K+ or NH4+ Rattus norvegicus
3.5.2.9 Mg2+
-
 Rattus norvegicus
3.5.2.9 Mn2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.9 325000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.9 5-oxo-L-proline + H2O + ATP Mus musculus
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Rattus norvegicus
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Sus scrofa
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Bos taurus
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Oryctolagus cuniculus
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Ovis aries
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Canis lupus familiaris
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Felis catus
-
 L-glutamate + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.9 Bos taurus
-
 cow
-
3.5.2.9 Canis lupus familiaris
-
 dog
-
3.5.2.9 Felis catus
-
 cat
-
3.5.2.9 Mus musculus
-
-
-
3.5.2.9 Oryctolagus cuniculus
-
 rabbit
-
3.5.2.9 Ovis aries
-
 sheep
-
3.5.2.9 Rattus norvegicus
-
-
-
3.5.2.9 Rattus norvegicus
-
 rat
-
3.5.2.9 Rattus norvegicus Sprague-Dawley
-
-
-
3.5.2.9 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.9
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.9 brain
-
 Mus musculus
-
3.5.2.9 brain
-
 Rattus norvegicus
-
3.5.2.9 brain
-
 Sus scrofa
-
3.5.2.9 brain
-
 Oryctolagus cuniculus
-
3.5.2.9 ciliary body
-
 Rattus norvegicus
-
3.5.2.9 ciliary body
-
 Oryctolagus cuniculus
-
3.5.2.9 kidney
-
 Mus musculus
-
3.5.2.9 kidney
-
 Rattus norvegicus
-
3.5.2.9 kidney
-
 Sus scrofa
-
3.5.2.9 kidney
-
 Bos taurus
-
3.5.2.9 kidney
-
 Oryctolagus cuniculus
-
3.5.2.9 kidney
-
 Ovis aries
-
3.5.2.9 kidney
-
 Canis lupus familiaris
-
3.5.2.9 kidney
-
 Felis catus
-
3.5.2.9 liver
-
 Mus musculus
-
3.5.2.9 liver
-
 Rattus norvegicus
-
3.5.2.9 liver
-
 Sus scrofa
-
3.5.2.9 liver
-
 Oryctolagus cuniculus
-
3.5.2.9 lung
-
 Rattus norvegicus
-
3.5.2.9 ovary
-
 Oryctolagus cuniculus
-
3.5.2.9 spleen
-
 Rattus norvegicus
-
3.5.2.9 spleen
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.9 0.88
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.5.2.9 4°C, 30 mM Tris-HCl buffer pH 7.8, 10 mM potassium chloride, 1 mM MgCl2, 5 mM 5-oxoproline, activity decreases to about 60% of its initial value after 2 days, 42% after 4 days and 40% after 5-6 days, addition of dithiothreitol leads to 104% of initial activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.9 2-imidazolidone-4-carboxylate + ATP + H2O
-
 Rattus norvegicus 2-imidazolidone + ADP + phosphate
-
 ?
3.5.2.9 2-piperidone-6-carboxylate + ATP + H2O
-
 Rattus norvegicus 2-aminoadipate + phosphate + ADP
-
 ?
3.5.2.9 3-oxy-5-oxoproline + H2O
-
 Rattus norvegicus 3-oxyglutamate + phosphate
-
 ?
3.5.2.9 4-oxy-5-oxoproline + H2O
-
 Rattus norvegicus 4-oxyglutamate + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Mus musculus L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Rattus norvegicus L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Sus scrofa L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Bos taurus L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Oryctolagus cuniculus L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Ovis aries L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Canis lupus familiaris L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Felis catus L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 ATP + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 ATP + H2O
-
 Rattus norvegicus Sprague-Dawley ?
-
 ?
3.5.2.9 dATP + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 dATP + H2O
-
 Rattus norvegicus Sprague-Dawley ?
-
 ?
3.5.2.9 GTP + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 GTP + H2O
-
 Rattus norvegicus Sprague-Dawley ?
-
 ?
3.5.2.9 ITP + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 ITP + H2O
-
 Rattus norvegicus Sprague-Dawley ?
-
 ?
3.5.2.9 additional information L-proline, 2-pyrrolidone, N-methyl-5-oxo-L-proline, DL-piperidine-2-carboxylic acid and cyclohexanone-3-carboxylic acid are no substrates Rattus norvegicus ?
-
 ?
3.5.2.9 additional information L-proline, 2-pyrrolidone, N-methyl-5-oxo-L-proline, DL-piperidine-2-carboxylic acid and cyclohexanone-3-carboxylic acid are no substrates Rattus norvegicus Sprague-Dawley ?
-
 ?
3.5.2.9 UTP + H2O
-
 Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.9 7
-
 second pH optimum at pH 9.7 Rattus norvegicus
3.5.2.9 9.7
-
 second pH optimum at pH 7.0 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.9 5.5 11.2
-
 Rattus norvegicus