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Literature summary extracted from

  • Seddon, A.P.; Li, L.; Meister, A.
    5-Oxo-L-prolinase from Pseudomonas putida (1985), Methods Enzymol., 113, 451-458.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.9 0.002
-
 L-2-oxothiazolidine-4-carboxylic acid
-
 Rattus norvegicus
3.5.2.9 0.002
-
 L-2-oxothiazolidine-4-carboxylic acid
-
 Pseudomonas putida
3.5.2.9 0.008
-
 5-oxo-L-proline
-
 Rattus norvegicus
3.5.2.9 0.008
-
 5-oxo-L-proline
-
 Pseudomonas putida
3.5.2.9 0.043
-
 5-oxo-L-proline
-
 Pseudomonas putida
3.5.2.9 0.15
-
 MgATP2-
-
 Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.9 Pseudomonas putida
-
-
-
3.5.2.9 Pseudomonas putida ALA
-
-
-
3.5.2.9 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.9
-
 Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.9 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.9 75
-
 component F8 Pseudomonas putida
3.5.2.9 243
-
 component beta5 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
3.5.2.9 4°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Rattus norvegicus L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP component F8 + component beta5 Pseudomonas putida L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 CTP + H2O less than 5% of the activity observed with ATP Pseudomonas putida ?
-
 ?
3.5.2.9 CTP + H2O less than 5% of the activity observed with ATP Pseudomonas putida ALA ?
-
 ?
3.5.2.9 dATP + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 dATP + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.9 dATP + H2O
-
 Pseudomonas putida ALA ?
-
 ?
3.5.2.9 GTP + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 GTP + H2O less than 5% of the activity observed with ATP Pseudomonas putida ?
-
 ?
3.5.2.9 GTP + H2O less than 5% of the activity observed with ATP Pseudomonas putida ALA ?
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Rattus norvegicus L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Pseudomonas putida L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylic acid + H2O
-
 Rattus norvegicus L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylic acid + H2O
-
 Pseudomonas putida L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 MgATP2- + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 MgATP2- + H2O component beta5 Pseudomonas putida ?
-
 ?
3.5.2.9 additional information no activity observed with 5-oxo-D-proline Pseudomonas putida ?
-
 ?
3.5.2.9 additional information no activity observed with 5-oxo-D-proline Pseudomonas putida ALA ?
-
 ?
3.5.2.9 UTP + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.9 UTP + H2O less than 5% of the activity observed with ATP Pseudomonas putida ?
-
 ?
3.5.2.9 UTP + H2O less than 5% of the activity observed with ATP Pseudomonas putida ALA ?
-
 ?
3.5.2.9 XTP + H2O less than 5% of the activity observed with ATP Pseudomonas putida ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.9 8.2
-
 component beta5, cleavage of ATP Pseudomonas putida
3.5.2.9 9.5
-
 component F8, ATP-dependent conversion of 5-oxoproline to glutamate Pseudomonas putida