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Literature summary extracted from

  • Siemann, M.; Alvarado-Marin, A.; Pietzsch, M.; Syldatk, C.
    A D-specific hydantoin amidohydrolase: properties of the metalloenzyme purified from Arthrobacter crystallopoietes (1999), J. Mol. Catal. , B Enzym., 6, 387-397.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.5.2.2 freezing of the enzyme lead to its subsequent inactivation Arthrobacter crystallopoietes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 8-Hydroxyquinoline-5-sulfonic acid
-
 Arthrobacter crystallopoietes
3.5.2.2 8-Hydroxyquinoline-5-sulfonic acid
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.2 7.9
-
 DL-5-phenylhydantoin
-
 Arthrobacter crystallopoietes
3.5.2.2 8
-
 phenylhydantoin
-
 Arthrobacter crystallopoietes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Zn2+
-
 Rattus norvegicus
3.5.2.2 Zn2+
-
 Paenarthrobacter aurescens
3.5.2.2 Zn2+
-
 Arthrobacter crystallopoietes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 60000
-
 4 * 60000, SDS-PAGE Arthrobacter crystallopoietes
3.5.2.2 247000
-
 calculated according to the repective amount of Zn2+ Paenarthrobacter aurescens
3.5.2.2 257000
-
 gel filtration Arthrobacter crystallopoietes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 5,6-dihydrothymine + H2O Rattus norvegicus
-
 ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O Pseudomonas putida
-
 ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O Arthrobacter crystallopoietes
-
 ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O Bacillus sp. (in: Bacteria) LU 1220
-
 ?
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Geobacillus stearothermophilus
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas fluorescens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas putida
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Bacillus sp. (in: Bacteria)
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Agrobacterium tumefaciens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Paenarthrobacter aurescens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Arthrobacter crystallopoietes
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Parageobacillus thermoglucosidasius
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Bacillus sp. (in: Bacteria) LU 1220
-
 3-ureidopropionate
-
 ?
3.5.2.2 hydantoin + H2O Homo sapiens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Rattus norvegicus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Geobacillus stearothermophilus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas fluorescens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Bacillus sp. (in: Bacteria)
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium tumefaciens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Arthrobacter crystallopoietes
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Parageobacillus thermoglucosidasius
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas sp. AJ-11220
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Agrobacterium sp.
-
-
-
3.5.2.2 Agrobacterium sp. IP-I 671
-
-
-
3.5.2.2 Agrobacterium tumefaciens
-
-
-
3.5.2.2 Arthrobacter crystallopoietes
-
 DSM 20117
-
3.5.2.2 Bacillus sp. (in: Bacteria)
-
 LU 1220
-
3.5.2.2 Bacillus sp. (in: Bacteria) LU 1220
-
 LU 1220
-
3.5.2.2 Geobacillus stearothermophilus
-
-
-
3.5.2.2 Homo sapiens
-
 human
-
3.5.2.2 Paenarthrobacter aurescens
-
-
-
3.5.2.2 Parageobacillus thermoglucosidasius
-
-
-
3.5.2.2 Pseudomonas fluorescens
-
 strain DSM 84
-
3.5.2.2 Pseudomonas putida
-
-
-
3.5.2.2 Pseudomonas sp.
-
-
-
3.5.2.2 Pseudomonas sp. AJ-11220
-
-
-
3.5.2.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Arthrobacter crystallopoietes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 100
-
-
 Arthrobacter crystallopoietes
3.5.2.2 216
-
 DL-5-phenylhydantoin as substrate Arthrobacter crystallopoietes

Storage Stability

EC Number Storage Stability Organism
3.5.2.2 5°C, phosphate buffer, can be stored under weakly alkaline conditions for at least several weeks, Arthrobacter crystallopoietes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrothymine + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O
-
 Arthrobacter crystallopoietes ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O
-
 Agrobacterium sp. IP-I 671 ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O
-
 Bacillus sp. (in: Bacteria) LU 1220 ?
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Rattus norvegicus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus stearothermophilus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas fluorescens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Bacillus sp. (in: Bacteria) 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium tumefaciens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium sp. 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Paenarthrobacter aurescens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Arthrobacter crystallopoietes 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Parageobacillus thermoglucosidasius 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium sp. IP-I 671 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Bacillus sp. (in: Bacteria) LU 1220 3-ureidopropionate
-
 ?
3.5.2.2 D-5-(2-methylthioethyl)hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-methionine
-
 ?
3.5.2.2 D-5-(2-methylthioethyl)hydantoin + H2O
-
 Arthrobacter crystallopoietes N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Bacillus sp. (in: Bacteria) N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Paenarthrobacter aurescens N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O strictly D-selective Arthrobacter crystallopoietes N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium sp. IP-I 671 N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Bacillus sp. (in: Bacteria) LU 1220 N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Bacillus sp. (in: Bacteria) N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Arthrobacter crystallopoietes N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Agrobacterium sp. IP-I 671 N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Bacillus sp. (in: Bacteria) LU 1220 N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Arthrobacter crystallopoietes ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Agrobacterium sp. IP-I 671 ?
-
 ?
3.5.2.2 DL-5-indolylmethylhydantoin + H2O
-
 Pseudomonas fluorescens L-tryptophan
-
 ?
3.5.2.2 DL-5-indolylmethylhydantoin + H2O
-
 Paenarthrobacter aurescens L-tryptophan
-
 ?
3.5.2.2 DL-5-indolylmethylhydantoin + H2O strictly D-selective Arthrobacter crystallopoietes L-tryptophan
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Rattus norvegicus N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Paenarthrobacter aurescens N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Arthrobacter crystallopoietes N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-thienylmethylhydantoin + H2O
-
 Arthrobacter crystallopoietes ?
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas fluorescens hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bacillus sp. (in: Bacteria) hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Parageobacillus thermoglucosidasius hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bacillus sp. (in: Bacteria) LU 1220 hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Homo sapiens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Rattus norvegicus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bacillus sp. (in: Bacteria) N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Arthrobacter crystallopoietes N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Parageobacillus thermoglucosidasius N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas sp. AJ-11220 N-carbamoyl glycine
-
 ?
3.5.2.2 additional information DL-5-phenylhydantoin is no substrate Paenarthrobacter aurescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 tetramer
-
 Rattus norvegicus
3.5.2.2 tetramer
-
 Geobacillus stearothermophilus
3.5.2.2 tetramer
-
 Pseudomonas fluorescens
3.5.2.2 tetramer
-
 Paenarthrobacter aurescens
3.5.2.2 tetramer 4 * 60000, SDS-PAGE Arthrobacter crystallopoietes

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.2 4 60
-
 Arthrobacter crystallopoietes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.2 50
-
-
 Agrobacterium sp.
3.5.2.2 50
-
 50% initial activity remains after 6 h, activity decreases within min by incubating at 60°C Arthrobacter crystallopoietes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 8
-
-
 Geobacillus stearothermophilus
3.5.2.2 8
-
-
 Pseudomonas fluorescens
3.5.2.2 8
-
-
 Arthrobacter crystallopoietes