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Literature summary extracted from

  • Joo-Ho, P.; Geun-Joong, K.; Seun-Goo, L.; Dong-Cheol, L.; Hak-Sung, K.
    Purification and characterization of thermostable D-hydantoinase from Bacillus thermocatenulatus GH-2 (1999), Appl. Biochem. Biotechnol., 81, 53-64.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.2.2 Uracil induces biosynthesis of enzyme Geobacillus thermocatenulatus

Application

EC Number Application Comment Organism
3.5.2.2 synthesis commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals Geobacillus stearothermophilus
3.5.2.2 synthesis commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals Geobacillus thermocatenulatus
3.5.2.2 synthesis thermostable enzyme with great biotechnological potential Geobacillus thermocatenulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.2 30.7
-
 p-hydroxyphenylhydantoin
-
 Geobacillus thermocatenulatus
3.5.2.2 47.2
-
 p-hydroxyphenylhydantoin
-
 Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Co2+
-
 Geobacillus stearothermophilus
3.5.2.2 Co2+
-
 Geobacillus thermocatenulatus
3.5.2.2 Fe2+
-
 Geobacillus thermocatenulatus
3.5.2.2 Mn2+
-
 Geobacillus stearothermophilus
3.5.2.2 Mn2+
-
 Geobacillus thermocatenulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 56000
-
 4 * 56000, SDS-PAGE Geobacillus thermocatenulatus
3.5.2.2 120000
-
 strain SD-1 Geobacillus stearothermophilus
3.5.2.2 200000
-
 strain NS1122A Geobacillus stearothermophilus
3.5.2.2 230000
-
 gel filtration Geobacillus thermocatenulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 hydantoin + H2O Geobacillus stearothermophilus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Geobacillus thermocatenulatus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Geobacillus stearothermophilus SD-1
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Agrobacterium sp.
-
-
-
3.5.2.2 Geobacillus stearothermophilus
-
-
-
3.5.2.2 Geobacillus stearothermophilus
-
 strains NS1122A and LU1220
-
3.5.2.2 Geobacillus stearothermophilus SD-1
-
-
-
3.5.2.2 Geobacillus thermocatenulatus
-
-
-
3.5.2.2 Geobacillus thermocatenulatus GH-2
-
-
-
3.5.2.2 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Geobacillus thermocatenulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 10.9
-
 isopropylhydantoin as substrate Geobacillus thermocatenulatus
3.5.2.2 27.5
-
 dihydrouracil as substrate Geobacillus thermocatenulatus
3.5.2.2 91.6
-
 hydroxyphenylhydantoin as substrate Geobacillus thermocatenulatus
3.5.2.2 101.5
-
 hydantoin as substrate Geobacillus thermocatenulatus
3.5.2.2 236.9
-
 phenylhydantoin as substrate Geobacillus thermocatenulatus
3.5.2.2 461
-
-
 Geobacillus thermocatenulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus stearothermophilus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium sp. 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus thermocatenulatus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus stearothermophilus SD-1 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus thermocatenulatus GH-2 3-ureidopropionate
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Geobacillus thermocatenulatus N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Geobacillus stearothermophilus SD-1 N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Geobacillus thermocatenulatus GH-2 N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-isobutyl-hydantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Geobacillus thermocatenulatus N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Geobacillus thermocatenulatus GH-2 N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Geobacillus thermocatenulatus N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Geobacillus stearothermophilus SD-1 N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Geobacillus thermocatenulatus GH-2 N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus thermocatenulatus hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus SD-1 hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus thermocatenulatus GH-2 hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus thermocatenulatus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus SD-1 N-carbamoyl glycine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 tetramer
-
 Geobacillus stearothermophilus
3.5.2.2 tetramer 4 * 56000, SDS-PAGE Geobacillus thermocatenulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.2 65
-
-
 Geobacillus stearothermophilus
3.5.2.2 65
-
-
 Geobacillus thermocatenulatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.2 30 80
-
 Geobacillus thermocatenulatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.2 80
-
-
 Geobacillus stearothermophilus
3.5.2.2 80
-
 half-life about 45 min, stable up to 75°C Geobacillus thermocatenulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 7.5
-
-
 Geobacillus thermocatenulatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.2 6 9.5
-
 Geobacillus thermocatenulatus