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Literature summary extracted from

  • Mukohara, Y.; Ishikawa, T.; Watabe, K.; Nakamura, H.
    A thermostable hydantoinase of Bacillus stearothermophilus NS1122A: Cloning, sequencing, and high expression of the enzyme gene, and some properties of the expressed enzyme (1994), Biosci. Biotechnol. Biochem., 58, 1621-1626.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.2.2 medicine
-
 Pseudomonas putida
3.5.2.2 medicine industrial production of L-amino acids Geobacillus stearothermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.2 gene for thermostable hydantoinase cloned and overexpressed in Escherichia coli JM105, vector pAHN134 Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 2,2'-dipyridyl
-
 Geobacillus stearothermophilus
3.5.2.2 2,2'-dipyridyl
-
 Pseudomonas putida
3.5.2.2 EDTA inhibition 43% Geobacillus stearothermophilus
3.5.2.2 nitrilotriacetic acid inhibition 42% Geobacillus stearothermophilus
3.5.2.2 o-phenanthroline
-
 Bos taurus
3.5.2.2 o-phenanthroline
-
 Geobacillus stearothermophilus
3.5.2.2 o-phenanthroline
-
 Pseudomonas putida
3.5.2.2 p-hydroxymercuribenzoate
-
 Geobacillus stearothermophilus
3.5.2.2 p-hydroxymercuribenzoate
-
 Pseudomonas putida
3.5.2.2 trans-1,2-cyclohexandiaminetetraacetic acid inhibition 42% Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Co2+
-
 Bos taurus
3.5.2.2 Co2+
-
 Geobacillus stearothermophilus
3.5.2.2 Mn2+
-
 Bos taurus
3.5.2.2 Mn2+
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 51720
-
 calculated from amino acid sequence Geobacillus stearothermophilus
3.5.2.2 52000
-
 4 * 52000, recombinant enzyme, SDS-PAGE Geobacillus stearothermophilus
3.5.2.2 60000
-
 2 * 60000 + 2 * 62000 + 1 * 110000, room temperature, 2-mercaptoethanol, SDS-PAGE Geobacillus stearothermophilus
3.5.2.2 62000
-
 2 * 60000 + 2 * 62000 + 1 * 110000, room temperature, 2-mercaptoethanol, SDS-PAGE Geobacillus stearothermophilus
3.5.2.2 110000
-
 SDS-PAGE, room temperature, 2-mercaptoethanol Geobacillus stearothermophilus
3.5.2.2 200000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 hydantoin + H2O Bos taurus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Geobacillus stearothermophilus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas sp. NS671
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Bos taurus
-
 bovine
-
3.5.2.2 Geobacillus stearothermophilus
-
 strains NS1122A and LU1220
-
3.5.2.2 Pseudomonas putida
-
-
-
3.5.2.2 Pseudomonas sp.
-
-
-
3.5.2.2 Pseudomonas sp. NS671
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2 recombinant enzyme Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O
-
 Bos taurus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus stearothermophilus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bos taurus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas sp. NS671 N-carbamoyl glycine
-
 ?
3.5.2.2 L-5-(2-methylthioethyl)hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 oligomer 2 * 60000 + 2 * 62000 + 1 * 110000, room temperature, 2-mercaptoethanol, SDS-PAGE Geobacillus stearothermophilus
3.5.2.2 tetramer
-
 Bos taurus
3.5.2.2 tetramer 4 * 52000, recombinant enzyme, SDS-PAGE Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.2 40 80
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.2 40 80 fairly thermostable, oligomer retains full activity at 40-60°C, still retains 98% initial activity at 70°C Geobacillus stearothermophilus